西湖龙井茶对高脂高果糖饮食小鼠肠道菌群的调节作用  

Regulatory effects of Xihu Longjing tea on the intestinal flora of mice fed with high-fat and high-fructose diet

作　　者：胡俐泉 屠琳玥 赵悦伶 谢亨通 徐安安 徐平[1] 周继红 王岳飞[1] HU Liquan;TU Linyue;ZHAO Yueling;XIE Hengtong;XU An’an;XU Ping;ZHOU Jihong;WANG Yuefei(Tea Research Institute,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学农业与生物技术学院茶叶研究所,浙江杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2024年第3期481-494,共14页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金区域创新发展联合基金项目(U19A2034)。

摘　　要：越来越多的研究表明,肠道菌群及其介导的微生态环境在高脂高果糖饮食引起的相关代谢障碍中发挥着关键作用。本研究首先探究了西湖龙井茶对常规饮食小鼠肠道菌群的作用,随后构建高脂高果糖饮食小鼠模型,进一步研究西湖龙井对预防高脂高果糖饮食小鼠脂质积累、肠道屏障功能损伤和肠道微生态紊乱的效果。结果表明:西湖龙井茶汤能够抑制高脂高果糖饮食小鼠的体质量上升和脂肪积累,有效改善代谢紊乱。进一步探究西湖龙井对高脂高果糖饮食小鼠肠道稳态的保护作用时发现,西湖龙井茶汤处理抑制了小鼠肠道上皮细胞低氧诱导因子-1α(hypoxia inducible factor-1α, HIF-1α)及其靶基因烯醇化酶-1(enolase-1, ENO1)和乳酸脱氢酶A(lactate dehydrogenase A, LDHA)的表达。此外,西湖龙井茶汤干预可以降低小鼠肠道厚壁菌门/拟杆菌门菌群丰度的比值,使norank_f_Muribaculaceae等益生菌富集,且高脂高果糖饮食+低浓度茶汤处理下毛螺菌科菌群富集,高脂高果糖饮食+高浓度茶汤处理使颤螺菌属菌群的相对丰度增加。本研究结果为龙井茶通过改善肠道菌群紊乱来调节脂肪积累提供了理论依据,为饮茶能够改善肠道健康、发挥降脂减肥作用提供了理论支持。An increasing number of studies have shown that the intestinal flora and microbial ecology mediated by the intestinal flora play key roles in metabolic disorders associated with high-fat and high-fructose diet.In this study,we investigated the effects of Xihu Longjing tea on the intestinal flora of mice fed with a conventional diet,followed by the construction of a mouse model with a high-fat and high-fructose diet to investigate the effects of Xihu Longjing on the prevention of lipid accumulation,intestinal barrier function impairment and intestinal microecological disorders in mice fed with high-fat and high-fructose diet.The results showed that Xihu Longjing tea soup inhibited the body mass gain and lipid accumulation in mice fed with a high-fat and high-fructose diet and effectively relieved metabolic disorders.When investigating the protective effects of Xihu Longjing on intestinal homeostasis in mice fed with a high-fat and high-fructose diet,it was found that Xihu Longjing tea soup treatment inhibited the expression of hypoxia inducible factor-1α(HIF-1α)and its target genes enolase-1(ENO1)and lactate dehydrogenase A(LDHA)in the intestinal epithelial cells of mice.In addition,Xihu Longjing tea soup intervention reduced the abundance of intestinal bacteria of Firmicutes/Bacteroidota ratio and enriched the abundance of probiotic bacteria such as norank_f_Muribaculaceae in mice,and then the high-fat and high-fructose diet with low concentration of Xihu Longjing treatment enriched Lachnospiraceae bacteria and the high-fat and high-fructose diet with high concentration of Xihu Longjing treatment increased the relative abundance of Oscillospirales bacteria.The results provide a theoretical basis for Longjing tea to regulate fat accumulation by improving intestinal flora disorders,and provide theoretical support for the ability of tea consumption to improve intestinal health and play a role in fat reduction and body mass loss.

关 键 词：西湖龙井茶 高脂高果糖饮食 肠道菌群 肠道屏障 

分 类 号：S571.1[农业科学—茶叶生产加工] R282.71[农业科学—作物学]