Mechanism of Anti-tumor Effects of Viola Medicinal Materials Based on Network Pharmacology and Molecular Docking  

作　　者：Xiaoyong HE Liniu SHAMA Dongmei SHA Shuaicong NI Jing WEN Xinjia YAN Yuan LIU 

机构地区：[1]College of Pharmacy,Southwest Minzu University,Chengdu 610041,China [2]Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory,Chengdu 610225,China [3]Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China,Chengdu 610225,China [4]School of Pharmacy,Sichuan College of Traditional Chinese Medicine,Mianyang 621000,China

出　　处：《Medicinal Plant》2024年第3期9-15,共7页药用植物：英文版

基　　金：Supported by the National Key Research and Development Plan (2018YFC1708005);Sichuan Provincial Natural Science Foundation (Young Scientist Fund Project-2022NSFSC1588);Leading Talent Support Plan of National Ethnic Affairs Commission in 2021,Double First-Class Initiative Project of Southwest Minzu University (CX2023054).

摘　　要：[Objectives]To explore the relationship between anti-tumor components,targets,and pathways involved in Viola medicinal materials,study its main active components,and evaluate its inhibitory activity.[Methods]Through network pharmacological analysis,molecular docking simulation experiments and in vitro experiments,the main components and corresponding targets of Viola were screened out,and their anti-tumor signaling pathways were confirmed.MTT colorimetric assay was used to investigate the inhibitory effect of different extraction layers of Viola on the growth of tumor cells.[Results]18 anti-tumor active components such as 2α,19α-Dihydroxyursolic acid,Corlumine,Madolin U,Trifolirhizin and etc.,and 52 action targets such as PTGS2,PTGS1,P2RX7,MMP1,and GABRB3,and anti-tumor signaling pathways were confirmed.The results of molecular docking showed that all the selected Viola compounds had good binding activity.The results of MTT colorimetric assay showed that the petroleum ether layer and n-butanol layer had a good inhibitory effect on the growth of tumor cell lines.[Conclusions]Viola medicinal materials have the potential of anti-tumor,triterpenoids and flavonoids may be the main active components,and the petroleum ether layer and n-butanol layer have better inhibitory effect on the growth of tumor cells.

关 键 词：VIOLA MEDICINAL materials ANTI-TUMOR Network PHARMACOLOGY Molecular DOCKING MTT 

分 类 号：R285[医药卫生—中药学]