脑缺血再灌注后补体C3高迁移率族蛋白B1及谷胱甘肽过氧化物酶4表达变化  

Changes in C3,HMGB1 and GPX4 expression in rats after cerebral ischemia-reperfusion injury

作　　者：朱涵婷 熊瑞芳 李承毅 赵锡海 唐小平[1] Zhu Hanting;Xiong Ruifang;Li Chengyi;Zhao Xihai;Tang Xiaoping(Department of Radiology,Second Affiliated Hospital,Nanchang University,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学第二附属医院医学影像中心,330006 [2]清华大学医学院生物医学影像研究中心

出　　处：《中华老年心脑血管病杂志》2024年第6期699-703,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：江西省自然科学基金(20232BAB206132)。

摘　　要：目的探讨大鼠脑缺血再灌注损伤后不同时间点脑组织补体C3、高迁移率族蛋白B1(HMGB1)、谷胱甘肽过氧化物酶4(GPX4)表达的变化。方法选择SPF级健康雄性SD大鼠72只,按数字随机法分为假手术组36只和模型组36只,线栓法制备大脑中动脉闭塞模型,根据再灌注时间分为3、6、24 h及3 d共4个时间点,每个时间点9只。参照Zea Longa评分法行神经功能学评分。每组行R2StarMap成像、弥散加权成像(DWI)、T_1加权成像、T_2加权成像扫描。测量DWI异常信号体积,检测脑梗死体积。R2StarMap原始图经后处理生成R2^(*)伪彩图,测量双侧大脑中动脉供血区R2^(*)值,检测脑铁沉积含量。采用蛋白免疫印迹法检测C3、HMGB1、GPX4表达。结果假手术组大鼠无神经功能损伤,模型组大鼠3、6、24 h及3 d神经功能评分较假手术组明显升高,差异有统计学意义(P<0.05)。模型组大鼠3、6、24 h及3 d右侧脑梗死体积较假手术组明显增大,差异有统计学意义(P<0.05)。模型组3、6、24 h及3 d右侧大脑中动脉供血区R2^(*)值明显高于左侧(P<0.05,P<0.01)。模型组3、6、24 h及3 d右侧大脑中动脉供血区R2^(*)值明显高于假手术组右侧(P<0.05,P<0.01)。模型组3、6、24 h及3 d的R2^(*)比值明显高于假手术组(1.82±0.82 vs 1.12±0.31,P<0.05;1.31±0.26 vs 1.04±0.14,P<0.05;1.94±0.74 vs 1.06±0.10,P<0.01;1.99±0.39 vs 1.02±0.11,P<0.01)。与假手术组比较,模型组3、6、24 h及3 d补体C3和HMGB1表达明显升高,GPX4表达明显降低,差异有统计学意义(P<0.01)。结论脑缺血再灌注损伤大鼠神经功能对脑组织中补体C3、HMGB1、GPX4表达有显著影响。Objective To investigate the changes in the expression of complement C3,high mobility group protein B1(HMGB1),and glutathione peroxidase 4(GPX4)in brain tissue at different time points after cerebral ischemia-reperfusion injury in rats.Methods A total 72 SPF male SD rats were randomly divided into a sham operation group(36 rats)and a model group(36 rats).A rat model of middle cerebral artery occlusion was established using thread occlusion,and then the rats from the model group were further assigned into 4 subgroups with reperfusion time for 3,6 and 24 h and 3 d,respectively,with 9 rats in each subgroup.Zea Longa scoring was used to assess neurological function.R2StarMap imaging,diffusion-weighted imaging(DWI),T_1 weighted imaging,and T_2 weighted imaging were performed on all the rats.The volume of abnormal DWI signals were detected and the volume of cerebral infarction was measured.The original R2StarMap images were post-processed to generate R2^(*)pseudo color images,and then the R2^(*)values of the areas with blood supply from bilateral middle cerebral artery were measured to detect iron deposition in the brain.The protein expression of C3,HMGB1,and GPX4 was detected by Western blotting.Results The sham operation group showed no neurological damage,while the model group had a significant increase in neurological function scores at 3,6 and 24 h and 3 d after modeling when compared to the sham surgery group(P<0.05).While the size of right cerebral infarct was increased significantly at 3,6 and 24 h and 3 d in the rats of the model group(P<0.05).The R2^(*)value of the area with blood supply from the right middle cerebral artery in the model group was significantly higher than that in the left side at 3,6 and 24 h and 3 d(P<0.05,P<0.01),and it was also obviously higher than that in the right side of the sham operation group at these time points(P<0.05,P<0.01).The R2^(*)ratio of the right and left blood supply areas in the model group at 3,6 and 24 h and 3 d was also statistically higher than that in the sham oper

关 键 词：再灌注损伤 补体C3 高迁移率族蛋白质类 谷胱甘肽过氧化酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]