三棱多糖RSB-4结构表征及抗炎活性研究  

Structural characterization and anti-inflammatory activity of polysaccharide RSB-4 from Sparganium stoloniferum

作　　者：张宁[1,2] 刘咏 张超[2] 王军辉 ZHANG Ning;LIU Yong;ZHANG Chao;WANG Junhui(Engineering Research Center of Bio-process of Ministry of Education,Hefei University of Technology,Hefei 230601,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)

机构地区：[1]合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230601 [2]合肥工业大学食品与生物工程学院,安徽合肥230601

出　　处：《合肥工业大学学报（自然科学版）》2024年第6期796-802,共7页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省重点研究与开发计划资助项目(2023n06020034)。

摘　　要：三棱是一种常见的草本植物,用作中药具有破血行气、消积止痛等功效。文章采用热水提取法从三棱根茎中提取出多糖RSB,再通过DEAE-Cellulose阴离子交换层析柱进一步分离纯化得到三棱多糖RSB-4;并对RSB-4结构进行表征,结果表明,三棱多糖RSB-4的平均分子量大小为9.32×10^(5) Da,单糖主要由葡萄糖组成,是一种葡聚糖。结合甲基化和核磁共振谱(nuclear magnetic resonance,NMR)分析结果可知,三棱多糖RSB-4的主链由(1→3)-α-D-Glc p组成,在(1→3)-α-D-Glc p的O-6位连有支链T-β-D-Glc p和(1→6)-α-D-Glc p。免疫活性研究结果表明,三棱多糖RSB-4显著提高了巨噬细胞RAW264.7的增殖活性并促进NO的释放。研究结果为三棱多糖在功能性食品、抗炎药物上的开发和应用提供了理论基础。Sparganium stoloniferum is a common herb,which has the effects of promoting blood circulation and relieving pain in traditional Chinese medicine.In this paper,polysaccharide RSB was obtained by hot water extraction from the rootstock of Sparganium stoloniferum.Then,polysaccharide RSB-4 was further purified by DEAE-Cellulose anion exchange chromatography.The structural characterization of RSB-4 was analyzed.The results showed that average molecular weight of polysaccharide RSB-4 was 9.32×10^(5) Da,and monosaccharides consist mainly of glucose,which showed that RSB-4 is a type of glucan.Combined the results of methylation with nuclear magnetic resonance(NMR)analysis,the main chain of RSB-4 consisted of(1→3)-α-D-Glc p,with branched T-β-D-Glc p and(1→6)-α-D-Glc p attached to the O-6 position of(1→3)-α-D-Glc p.Anti-inflammatory activity studies showed that polysaccharide RSB-4 significantly increased the proliferative activity of macrophage RAW264.7 and promoted the release of NO.The results provide a theoretical basis for the development and application of Sparganium stoloniferum polysaccharides in functional foods and anti-inflammatory drugs.

关 键 词：三棱 多糖 理化性质 结构表征 抗炎活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]