α5-nAChR与MHC-I在肺腺癌中的表达及相关性  

Expression and correlation ofα5-nAChR and MHC-I in lung adenocarcinoma

作　　者：王靖婷 王璟 鲁艺 李静坦 李强 郏雁飞 马晓丽 WANG Jingting;WANG Jing;LU Yi;LI Jingtan;LI Qiang;JIA Yanfei;MA Xiaoli(Research Center of Basic Medicine,Central Hospital Affiliated to Shandong First Medical University,Jinan 250013,Shandong,China)

机构地区：[1]山东第一医科大学附属中心医院基础医学研究中心,山东济南250013

出　　处：《山东大学学报（医学版）》2024年第5期72-78,共7页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金面上项目(ZR2021MH322)。

摘　　要：目的 探讨α5-烟碱型乙酰胆碱受体(alpha5-nicotinic acetylcholine receptor, α5-nAChR)与主要组织相容性复合体Ⅰ类分子(major histocompatibility complex class I molecule, MHC-I)在肺腺癌中的表达及相关性。方法 TCGA数据库分析CHRNA5(编码α5-nAChR基因)与HLA-B(编码MHC-I基因)在肺腺癌中的表达、相关性及其临床意义。运用免疫组织化学技术检测人肺腺癌临床样本、裸鼠肺腺癌异种移植瘤组织中α5-nAChR与MHC-I的表达及相关性。在人A549细胞、小鼠LLC细胞中,Western blotting检测在α5-nAChR不同表达水平下的FHIT与MHC-I表达及相关性。结果 数据库分析表明,CHRNA5高表达或HLA-B低表达肺腺癌患者的生存率降低,CHRNA5与HLA-B的表达呈负相关(P<0.05)。α5-nAChR与MHC-I在人肺腺癌、裸鼠肺腺癌异种移植物中的表达呈负相关(P<0.05)。在肺腺癌细胞中,α5-nAChR的表达分别与FHIT、MHC-I的表达呈负相关(P<0.05),FHIT和MHC-I在肺腺癌细胞中的表达呈正相关(P<0.05)。结论 α5-nAChR与MHC-I的表达呈负相关,并参与肺腺癌的发生。Objective To investigate the expression and correlation between alpha5-nicotinic acetylcholine receptor(α5-nAChR)and the major histocompatibility complex class I molecule(MHC-I)in lung adenocarcinoma.Methods TCGA database were used to study the expression,correlation,and clinical significance of encodingα5-nAChR gene(CHRNA5)and encoding MHC-I gene(HLA-B)in lung adenocarcinoma.The expressions ofα5-nAChR and MHC-I were tested by immunohistochemistry staining in human lung adenocarcinoma specimans and nude mouse lung adenocarcinoma xenografts tissues.Furthermore,the expression and correlation of FHIT and MHC-I at different expression levels ofα5-nAChR were detected by Western blotting in human A549 cells and mice LLC cells.Results Patients with lung adenocarcinoma with high expression of CHRNA5 or low expression of HLA-B had reduced survival,and CHRNA5 was negatively correlated with the expression of HLA-B(P<0.05).The expressions ofα5-nAChR and MHC-I were negatively correlated in human lung adenocarcinoma and nude mouse lung adenocarcinoma xenografts tissues(P<0.05).In lung adenocarcinoma cells,the expression ofα5-nAChR was negatively correlated with the expressions of FHIT and MHC-I,and the expression of FHIT and MHC-I was positively correlated(P<0.05).Conclusion The expressions ofα5-nAChR and MHC-I are negatively correlated and involved in lung adenocarcinogenesis.

关 键 词：α5-烟碱型乙酰胆碱受体 脆性组氨酸三联体 主要组织相容性复合体I类分子 肺腺癌 相关性 

分 类 号：R574[医药卫生—消化系统]