大蝎子草总多酚提取工艺优化及抗氧化活性  

Optimization of Extraction Process and Antioxidant Activity of Total Polyphenols from Girardinia diversifolia

作　　者：高林晓 石景海 勾娜 伍泽波 管永庆 郭蒙 GAO Linxiao;SHI Jinghai;GOU Na;WU Zebo;GUAN Yongqing;GUO Meng(School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Duyun,Guizhou 558000,China)

机构地区：[1]黔南民族师范学院化学化工学院,贵州都匀558000

出　　处：《家畜生态学报》2024年第6期50-56,共7页Journal of Domestic Animal Ecology

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]440,[2015]389);国家级大学生创新创业训练项目(202110670005);黔南民族师范学院校级科研项目(2020qnsy14);贵州省普通高校民族药用植物资源开发工程研究中心开放基金(黔教合KY字[2014]227)。

摘　　要：本试验采用超声辅助乙醇提取大蝎子草地上部分总多酚,通过单因素试验结果设计Box-Behnken模型,优化大蝎子草地上部分总多酚最佳工艺条件,用DPPH自由基清除率、FRAP法评价大蝎子草地上部分总多酚的抗氧化性。结果显示,乙醇浓度、液固比、超声时间、超声温度的最佳工艺参数分别为75%、50 mL/g、35min、40℃时,总多酚提取率为2.81%,与Box-Behnken模型的预测值相符。抗氧化性试验表明,0.15 mg/mL大蝎子草总多酚提取液DPPH清除率可达75.8%,半数抑制浓度IC_(50)值为0.057 mg/mL,具有一定的抗氧化性,为大蝎子草野生资源的研究和利用提供相应的理论支撑,为新型饲料添加剂的开发提供理论基础。The ultrasonic-assisted ethanol was used to extract the total polyphenols from the aerial part of Girardinia diversifolia in this experiment.Box-Behnken model was designed based on the results of single-factor experiments to optimize the optimal process conditions of total polyphenols from the aerial part of Girardinia diversifolia.DPPH free radical scavenging rate and FRAP method were used to evaluate the antioxidant capacity of total polyphenols from the aerial part of Girardinia diversifolia.Results showed when the optimal process parameters as ethanol concentration,liquid-solid ratio,ultrasonic time and ultrasonic temperature were 75%,50 mL/g,35 min and 40℃,respectively,the extraction rate of total polyphenols was 2.81%,which was consistent with the predicted value of Box-Behnken model.The antioxidant test showed that the DPPH radical-scavenging activity of 0.15 mg/mL total polyphenol from the aerial part of Girardinia diversifolia could reach 75.8%,and the IC_(50) value of the half inhibitory concentration was 0.057 mg/mL,which had certain antioxidant capacity.It provides corresponding theoretical data support for further research and utilization of wild resources of Girardinia diversifolia,and a theoretical basis for the development of new feed additives.

关 键 词：总多酚 大蝎子草 超声提取 抗氧化 

分 类 号：S812.8[农业科学—草业科学] R248.2[农业科学—畜牧学]