磷酸化蛋白质的定量与化学计量分析方法的研究进展  

Advances in Quantification and Site Stoichiometry Analysis Methods for Phosphorylated Proteins

作　　者：刘媛 翟睿[2] 吴帆 楚占营 赵洋 戴新华 方向[2] 俞晓平[1] LIU Yuan;ZHAI Rui;WU Fan;CHU Zhan-Ying;ZHAO Yang;DAI Xin-Hua;FANG Xiang;YU Xiao-Ping(Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine,College of Life Sciences,China Jiliang University,Hangzhou 310018,China;National Market Supervision Technology Innovation Center(Mass Spectrometry),Frontier Metrology Science Center,Chinese Academy of Metrology,Beijing 100029,China)

机构地区：[1]中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州310018 [2]中国计量科学研究院前沿计量科学中心,国家市场监管技术创新中心(质谱),北京100029

出　　处：《分析化学》2024年第5期609-623,共15页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划项目(No.2022YFF0608400);国家自然科学基金项目(No.21927812);中国计量科学研究院基本科研业务费项目(Nos.AKY1955,AKY1941,AKY1942)资助。

摘　　要：蛋白质翻译后修饰是赋予蛋白质生理功能的关键机制,其中可逆的磷酸化修饰在众多生命活动中起着开/关的作用,其异常变化通常与多种重大疾病过程密切相关。近年来,借助蛋白质组学技术和方法,磷酸化蛋白质的高通量、高精度定性与定量分析方法也得到了快速发展。本文综述了近年来基于“自下而上”策略的磷酸化蛋白质定量与化学计量分析方法的研究进展,包括磷酸化肽的富集方法、磷酸化肽质谱碎裂方式、定量分析方法以及磷酸化位点的化学计量分析方法,并对磷酸化蛋白质研究的发展趋势进行了讨论。The post-translational modification of proteins is a key mechanism that imparts physiological functions to proteins,among which reversible phosphorylation modifications play a pivotal role in many biological processes.Aberrant changes in phosphorylation are often closely associated with various major disease processes.In recent years,with the aid of proteomic technologies and methods,high-throughput,high-precision qualitative and quantitative approaches for phosphorylated proteins have rapidly advanced.This article reviews the research progress of phosphorylated protein quantification and chemical proteomics analysis methods based on the"bottomup"strategy,including phosphopeptide enrichment methods,mass spectrometry fragmentation methods,quantification analysis methods and phosphorylation site stoichiometry,and discusses the development trend of quantification and stoichiometric analysis methods for phosphorylated proteins.

关 键 词：磷酸化蛋白质 富集方法 质谱 定量方法 化学计量 评述 

分 类 号：TB99[一般工业技术—计量学] Q503[机械工程—测试计量技术及仪器]