茶树种质叶片色素含量与叶色参数的关系  

Relationship between leaf pigment content and leaf color parameters in tea tree germplas

作　　者：宁秋燕 冯亚颀 何卫中 吉庆勇 疏再发 郑生宏 NING Qiuyan;FENG Yaqi;HE Weizhong;JI Qingyong;SHU Zaifa;ZHENG Shenghong(Lishui Academy of Agricultural and Forestry Sciences,Lishui,Zhejiang 323000,China)

机构地区：[1]丽水市农林科学研究院,浙江丽水323000

出　　处：《西北植物学报》2024年第6期870-879,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：浙江省农业(茶树)新品种选育重大科技专项(2021C02067-6);中国农业科学院茶叶研究所长三角创新中心项目建设(SYC-ZHT-01);2022年市级农科院联盟区域示范性项目(2022SJLM20)。

摘　　要：[目的]探究茶树种质资源叶片色素含量与叶色参数之间的关系,为叶色特异茶树种质资源呈色机理探索和品种选育提供理论依据。[方法]以浙江省茶树种质资源圃中143份黄化、白化和紫化叶色特异茶树种质春季新梢为材料,比较分析各种质叶片叶色参数L^(*)、a^(*)、b^(*)值和花青素、叶绿素、类胡萝卜素含量差异。通过主成分分析、聚类分析、相关性分析、多元逐步回归分析和通径分析探讨叶片色素含量和叶色参数关系。[结果](1)紫化茶树种质花青素含量和a^(*)值较高,叶片呈现紫红色;黄化种质类胡萝卜素含量/叶绿素含量比值和b^(*)值较高,叶片呈黄色,白化种质L^(*)值较高,叶片呈白色;黄化和白化茶树种质叶绿素含量较低。在叶色表型性状方面,叶色特异茶树种质大体聚为两大类群,白色系和黄色系聚为一大类,紫色系聚为另一大类。(2)叶色特异茶树种质叶片叶色参数a^(*)值与花青素含量呈极显著正相关,L^(*)值、b^(*)值与花青素和叶绿素含量均呈极显著负相关,且b^(*)值与类胡萝卜素含量呈极显著正相关;花青素、叶绿素对叶片L^(*)、a^(*)、b^(*)值直接作用较大。[结论]a^(*)值可作为描述叶色特异茶树种质叶片紫红色性状的代表性参数,b^(*)值可作为描述叶色特异茶树种质叶片黄色性状的代表性参数。[Objective]This study aims to investigate the relationship between leaf pigment content and leaf color parameters of tea germplasm resources,and to provide theoretical basis for the exploration of leaf color presentation mechanism as well as variety selection and breeding of leaf color-specific tea germplasm resources.[Methods]The spring shoots of 143 leaf color-specific tea germplasms with yellow,albino,and purple variation were used as materials from the Tea Germplasm Resource Nursery of Zhejiang Province.The value of L^(*),a^(*),and b^(*)of leaf color parameters and the pigment content of total anthocyanin,chlorophyll,and carotenoid were analyzed.Through principal component analysis,cluster analysis,correlation analysis,multiple stepwise regression analysis,and pathway analysis,the relationship between leaf pigment content and leaf color parameters was explored.[Results](1)The purple tea tree germplasms had higher anthocyanin content and a^(*)value,and the leaves showed purple-red color.The ratio of carotenoid content to chlorophyll content and b^(*)value in yellow germplasms were higher,and the color of leaves were yellow;the albino germplasms had higher L^(*)value,and the color of leaves were white;and the chlorophyll content of the yellow and albino tea tree germplasms was lower.For leaf color phenotypic traits,the leaf color specific tea tree germplasms were generally clustered into two groups:the white and yellow series in one group,and the purple series in another group.(2)The leaf color parameter a^(*)value of the leaf color specific tea tree germplasms exhibited highly significant positively correlation with anthocyanin content,while L^(*)value as well as b^(*)value had highly significant negatively correlation with anthocyanin and chlorophyll content;the b^(*)value was highly significant positively correlated with the content of carotenoid.Anthocyanin and chlorophyll had a greater direct effect on the value of the leaf L^(*),a^(*),b^(*).[Conclusion]The a^(*)value can be used for describing the leaf pu

关 键 词：茶树 叶色 特异种质 色素含量 叶色参数 相关性 

分 类 号：S571.1[农业科学—茶叶生产加工]