Duplicated chalcone synthase(CHS)genes modulate flavonoid production in tea plants in response to light stress  

作　　者：Mingzhuo Li Wenzhao Wang Yeru Wang Lili Guo Yajun Liu Xiaolan Jiang Liping Gao Tao Xia 

机构地区：[1]State Key Laboratory of Tea Plant Biochemistry and Utilization,Anhui Agricultural University,Hefei 230036,China [2]School of Life Sciences,Anhui Agricultural University,Hefei 230036,China [3]College of Horticulture,Northwest A&F University,Yangling 712100,China [4]Department of Plant and Microbial Biology,North Carolina State University,Raleigh 27606,NC,USA

出　　处：《Journal of Integrative Agriculture》2024年第6期1940-1955,共16页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(U21A20232,32372756,and 32202551).

摘　　要：In tea plants,the abundant flavonoid compounds are responsible for the health benefits for the human body and define the astringent flavor profile.While the downstream mechanisms of flavonoid biosynthesis have been extensively studied,the role of chalcone synthase(CHS)in this secondary metabolic process in tea plants remains less clear.In this study,we compared the evolutionary profile of the flavonoid metabolism pathway and discovered that gene duplication of CHS occurred in tea plants.We identified three CsCHS genes,along with a CsCHS-like gene,as potential candidates for further functional investigation.Unlike the CsCHS-like gene,the CsCHS genes effectively restored flavonoid production in Arabidopsis chs-mutants.Additionally,CsCHS transgenic tobacco plants exhibited higher flavonoid compound accumulation compared to their wild-type counterparts.Most notably,our examination of promoter and gene expression levels for the selected CHS genes revealed distinct responses to UV-B stress in tea plants.Our findings suggest that environmental factors such as UV-B exposure could have been the key drivers behind the gene duplication events in CHS.

关 键 词：TEA flavonoids biosynthesis CHS gene duplication UV-B stress 

分 类 号：S571.1[农业科学—茶叶生产加工]