原发系统型间变性大细胞淋巴瘤中TOP2A基因及蛋白表达的研究  

Abnormality of TOP2A gene and its expression in anaplastic large cell lymphoma

作　　者：刘然 王晓红[1] 于冉 宫丽平[3] LIU Ran;WANG Xiao-hong;YU Ran;GONG Li-ping(Department of Pathology,Beijing Pinggu Hospital,Beijing 101200,China;Department of Basic Medicine,Weifang Nursing Vocational College,Shandong,Weifang 261000,China;Department of Pathology,Capital Medical University,Beijing 100069,China)

机构地区：[1]北京市平谷区医院病理科,北京101200 [2]潍坊护理职业学院基础医学院,山东潍坊261000 [3]首都医科大学基础医学院病理学系,北京100069

出　　处：《诊断病理学杂志》2024年第3期221-225,230,共6页Chinese Journal of Diagnostic Pathology

摘　　要：目的研究原发系统型间变性大细胞淋巴瘤(ALCL)中TOP2A基因改变与TOP2A蛋白的表达,并阐述其临床病理学意义。方法收集58例ALCL患者蜡块,运用免疫组化技术及免疫荧光原位杂交(FISH)技术分别检测TOP2A蛋白表达及TOP2A基因拷贝数的变化,并做相关统计。结果TOP2A蛋白阳性表达者约70%(41/58例),TOP2A基因扩增者约50%(26/50例)。TOP2A蛋白表达及TOP2A基因扩增同临床分期Ⅲ~Ⅳ、IPI评分高危组及Ki-67增殖指数较高组成正相关(P<0.05)。结论TOP2A与ALCL的发生、发展及肿瘤的增殖密切相关,TOP2A蛋白阳性表达及TOP2A基因扩增的患者生存时间反而较长。Objective To investigate TOP2A gene copy number alterations and protein expression in anaplastic large cell lymphoma(ALCL),and to further analyze related clinical and pathological significance.Methods Fifty-eight cases of ALCL tissues were collected.Immunohistochemistry and fluorescence in situ hybridization(FISH)were used to detect the expression of TOP2A protein and the genetic abnormality of TOP2A,respectively and corresponding relationship was analyzed.Results Among the 58 ALCL patients,41 cases were positive for TOP2A protein.Among 50 cases of ALCL,26 cases had TOP2A gene amplification.TOP2A protein and TOP2A gene amplification were associated with Ann Arbor stage,IPI score and Ki-67 Index(P<0.05).Conclusion TOP2A is closely related to the occurrence and development of ALCL and the proliferation of tumor.Patients with positive expression of TOP2A protein and amplification of TOP2A gene have longer survival time.

关 键 词：间变性大细胞淋巴瘤 拓扑异构酶Ⅱα 免疫组化 荧光原位杂交 

分 类 号：R733.4[医药卫生—肿瘤]