干扰Gal-1通过TGF-β通路抑制人乳腺癌MDA-MB-231细胞的EMT和迁移  

Interference with Gal-1 inhibits EMT and migration of human breast cancer MDA-MB-231 cells via TGF-βpathway

作　　者：任世忠 秦旭勇 周国利[1] 赵薇 曹淑俊 苗振宇 李成萍 REN Shizhong;QIN Xuyong;ZHOU Guoli;ZHAO Wei;CAO Shujun;MIAO Zhenyu;LI Chengping(College of Life Sciences,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城大学生命科学学院,山东聊城252000

出　　处：《中国病理生理杂志》2024年第6期1078-1084,共7页Chinese Journal of Pathophysiology

基　　金：山东省自然科学基金资助项目(No.ZR2020QC064);聊城大学科技处博士科研启动资金(No.318051604)。

摘　　要：目的:探究干扰半乳糖凝集素1(Gal-1)通过转化生长因子β(TGF-β)途径对人乳腺癌MDA-MB-231细胞上皮-间充质转化(EMT)、迁移及增殖的影响和作用机制。方法:以shGal-1稳转细胞株和对照细胞株为材料,用Western blot检测TGF-β处理后shGal-1对MDA-MB-231细胞EMT进程的影响;通过细胞划痕实验和Transwell实验检测TGF-β处理后shGal-1对细胞迁移和侵袭的影响;用Western blot检测shGal-1对TGF-β通路蛋白的影响;用MTT实验和Western blot检测shGal-1对细胞增殖的影响。结果:shGal-1可抑制TGF-β介导的MDA-MB-231细胞EMT,并降低ERK、AKT和GSK3β的磷酸化水平,同时shGal-1可抑制MDA-MB-231细胞的迁移、侵袭和增殖。结论:shGal-1可抑制TGF-β介导的MDA-MB-231细胞EMT、迁移和增殖。AIM:To explore the effect and mechanism of the interfering Gal-1 on epithelial-mesenchymal transition(EMT),migration and proliferation in MDA-MB-231 cells via transforming growth factor-β(TGF-β)pathway.METHODS:The stable cell lines(shGal-1)which Gal-1 expression were inhibited completely and their control cell lines were used as experimental cells.Western blot assay was used to detect the effects of shGal-1 on EMT process of MDA-MB-231 cells after TGF-βtreatment;The effect of shGal-1 on cell migration and invasion after TGF-βtreatment was verified by cell scratch and transwell test;The effect of shGal-1 on the TGF-βpathway related proteins were detected by western blot;Finally,the effect of shGal-1 on cell proliferation was detected by MTT and western blot.RESULTS:shGal-1 inhibited TGF-β-mediated EMT in MDA-MB-231 cells and regulated phosphorylation of pathway signaling molecules(ERK,AKT and GSK3β);shGal-1 could inhibit the proliferation of MDA-MB-231 cells.CONCLUSION:shGal-1 can inhibit the TGF-β-mediated EMT,migration and proliferation of MDA-MB-231 cells.

关 键 词：半乳糖凝集素1 上皮-间充质转化 转化生长因子Β MDA-MB-231细胞 细胞迁移 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤] R363.2[医药卫生—临床医学] Q253[生物学—细胞生物学]