高效液相色谱法测定食品中溴硝醇残留  

Determination of Bronopol Residue in Foods by High Performance Liquid Chromatography

作　　者：彭晓俊 梁伟华 秦汉 PENG Xiaojun;LIANG Weihua;QIN Han(Jiangmen Customs District Technology Center,Jiangmen 529100,China)

机构地区：[1]江门海关技术中心,广东江门529100

出　　处：《现代食品》2024年第9期142-145,共4页Modern Food

基　　金：海关总署科技项目(2023HK133)。

摘　　要：建立高效液相色谱法测定食品中溴硝醇残留的分析方法。样品经乙醇提取,0.22μm有机滤膜过滤,Agilent ZORBAX Eclipse XDB-C18色谱柱分离,高效液相色谱法检测。结果表明,在优化实验条件下,溴硝醇在0.02~10.00μg·mL^(-1)具有良好的线性关系,相关系数大于0.9999。在0.050μg·mL^(-1)、0.200μg·mL^(-1)、2.500μg·mL^(-1)共3个添加水平下,回收率为96.3%~106.0%,相对标准偏差为3.2%~5.8%。该法简单、快速、准确、灵敏度高,可应用于粮食、水果和蔬菜中溴硝醇残留的快速检测。A method for determination of bronopol residue in foods by high performance liquid chromatography was established.The samples were extracted by ethanol,filtered by 0.22μm organic filter membrane,separated by Agilent ZORBAX Eclipse XDB-C18 column,and detected by high-performance liquid chromatography.Under the optimized experimental conditions,there was a good linear relationship in the range of 0.02~10.00μg·mL^(-1) with correlation coefficient greater than 0.9999.The recoveries on the sample at 3 levels of addition were 96.3%~106.0%,with the relative standard deviations of 3.2%~5.8%.With the advantages of easy and rapid operation,accuracy and high sensitivity,the method was be applied to the determination of bronopol residues in grains,fruits and vegetables.

关 键 词：高效液相色谱 食品 溴硝醇 甲醇提取 

分 类 号：S481.8[农业科学—农药学]