高效液相色谱法测定年糕中二氧化硫脲  

Determination of Thiourea Dioxide in Rice Cake by High Performance Liquid Chromatograph

作　　者：周宁菱 徐美丽 孙盼 吴一明 吕舟博 程晓芳 刘思萌 黄永芳 ZHOU Ningling;XU Meili;SUN Pan;WU Yiming;LÜZhoubo;CHENG Xiaofang;LIU Simeng;HUANG Yongfang(Jingdezhen Market and Quality Supervision and Management Comprehensive Inspection and Testing Center,Jingdezhen 333000,China)

机构地区：[1]景德镇市市场监督管理综合检验检测中心,江西景德镇333000

出　　处：《现代食品》2024年第9期202-205,219,共5页Modern Food

基　　金：江西省市场监督管理局科技项目(GSJK202123)。

摘　　要：以Amide柱(250 mm×4.6 mm,3.5μm)为分离柱,以80%乙腈-水溶液为流动相,进行梯度洗脱,在波长278 nm处检测,建立高效液相色谱法测定年糕中二氧化硫脲含量的分析方法。结果表明,二氧化硫脲在0.2~50.0 mg·L^(-1)呈良好的线性关系,检出限为4.00 mg·kg^(-1),加标回收率为90.08%~96.73%,相对标准偏差不大于8.61%。该方法简便快速,实用性强,可用于年糕食品中二氧化硫脲的测定。The Amide column(250 mm×4.6 mm,3.5μm)was used as stationary phase,gradient elution was performed using 80%acetonitrile aqueous solution as the mobile phase,and detection was performed at a wavelength of 278 nm,to establish an analytical method for determining the content of thiourea dioxide in rice cakes using high�performance liquid chromatography.The results indicated that,the thiourea dioxide exhibited excellent linearity in the range of 0.2~50.0 mg·L^(-1),the detection limit was 4.00 mg·kg^(-1).The recovery rates were over the range of 90.08%~96.73%,the relative standard deviation was less than 8.61%.This method is simple,fast,and highly practical,and can be used for the determination of thiourea dioxide in rice cake food.

关 键 词：年糕 高效液相色谱 二氧化硫脲 分析 

分 类 号：O657.7[理学—分析化学]