植物乳杆菌及其后生元对育成期母貂生长性能、免疫功能及肠道健康的影响  

Effects of Lactobacillus plantarum and Lactobacillus plantarum Postbiotics on Growth Performance,Immune Status and Intestinal Microflora Health of Growing Female Minks

作　　者：李亚霖 甄士博 曹林 孙逢雪 王利华[1] LI Yalin;ZHEN Shibo;CAO Lin;SUN Fengxue;WANG Lihua(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《畜牧兽医学报》2024年第6期2530-2539,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省现代农业特种经济动物产业技术体系(SDAIT-21-8)。

摘　　要：旨在研究植物乳杆菌及其后生元对育成期母貂生长性能、免疫功能及肠道健康的影响。试验采用2×2试验设计,选取40只12周龄的灰色母貂分成4组,每组10个重复。4组分别为对照组、0.1%植物乳杆菌(活菌数>10^(6) CFU·mL^(-1))添加组、0.3%植物乳杆菌后生元添加组、0.1%植物乳杆菌+0.3%植物乳杆菌后生元添加组。预试期1周,正试期8周。试验期间记录体重和采食量用于测定生长性能,试验结束时采集血液、空肠组织和内容物检测血清免疫、黏膜免疫和肠道菌群等指标,结果发现:1)植物乳杆菌显著增加了水貂4周和8周的体重(P<0.05),提高了0~4周和0~8周的平均日增重、平均日采食量(P<0.05),降低了血清IgG、黏膜TNF-α的含量(P<0.05),增加空肠黏膜sIgA含量(P<0.05)。2)植物乳杆菌后生元显著增加血清IgG、空肠黏膜IFN-γ的含量(P<0.05),降低了IL-2、IL-12和TNF-α的含量(P<0.05)。3)植物乳杆菌及其后生元对育成期母貂肠道菌群的Alpha多样性指数影响不显著(P>0.05)。在属水平上,植物乳杆菌组的Sporosarcina、Aminobacter、Agathobacter的相对丰度显著低于未添加植物乳杆菌组(P<0.05),Fastidiosipila的相对丰度显著高于未添加植物乳杆菌组(P<0.05);植物乳杆菌后生元组Kocuria、Plesiomonas、unclassified_f_Lachnospiraceae、Sanguibacter、Microbacterium、Glutamicibacter和Paracoccus的相对丰度显著低于未添加后生元组(P<0.05),Staphylococcus、Weissella、Brevibacterium、Dietzia、Brachybacterium、Carnobacterium、Aerococcus和Sphingomonas的相对丰度显著高于未添加后生元组(P<0.05)。4)植物乳杆菌益生菌及后生元对育成期母貂血清中IgA、IgM含量和黏膜中IL-2、IL-10、IL-8、TNF-α含量影响互作效应显著(P<0.05)。综上所述,饲粮中添加植物乳杆菌益生菌可提高母貂的生长性能。植物乳杆菌益生菌及后生元均能够改善水貂的免疫功能,提高肠道有益菌属的相对丰度。This experiment was conducted to study the effects of Lactobacillus plantarum and Lactobacillus plantarum postbiotics on production growth performance,immune status and intestinal microflora health of growing female minks.A total of 40 gray female minks at 12 weeks of age were selected and divided into 4 groups with 10 replicates per group.The minks were fed basal diet,basal diet supplemented with 0.1%Lactobacillus plantarum(viable bacteria number>106 CFU·mL^(-1)),basal diet supplemented with 0.3%postbiotics of Lactobacillus plantarum,basal diet supplemented with 0.1%Lactobacillus plantarum and 0.3%postbiotics of Lactobacillus plantarum,respectively.The pre-experimental period lasted for 1 week,and the experimental period lasted for 8 weeks.Growth performance,serum immunity,mucosal immunity and intestinal microflora were tested in this experiment,and the results During the experiment,bodyweight and feed intake were recorded to determine the growth performance.At the end of the experiment,blood,jejunum tissue,jejunal content were collected to determine the immune indexes and microflora.The results were showed as follows:1)Compared to the minks in group without Lactobacillus plantarum,minks in Lactobacillus plantarum group had greater body weight at week 4 and week 8(P<0.05),average daily gain and average daily feed intake for the first 4 weeks and the entire 8 weeks(P<0.05),sIgA content in jejunal mucosa(P<0.05),and had less IgG content in serum and TNF-αcontent in jejunal mucosa(P<0.05).2)Minks supplemented with Compared to the minks in group without Lactobacillus plantarum postbiotics,minks in postbiotics had greater IgG content in serum and IFN-γcontent in jejunal mucosa(P<0.05),and less IL-2,IL-12 and TNF-αcontent in jejunal mucosa(P<0.05).3)Neither Lactobacillus plantarum nor postbiotics had significant effects on Alpha diversity index of intestinal flora for growing female minks(P>0.05).Minks in Lactobacillus plantarum group had less proportion of Sporosarcina,Aminobacter and Agathobacter,and greater pro

关 键 词：植物乳杆菌 植物乳杆菌后生元 水貂 生长性能 免疫功能 肠道健康 

分 类 号：S862.220.4[农业科学—野生动物驯养]