阿霉素处理后对犬乳腺肿瘤细胞系CHMp lncRNAs差异表达的影响  

The Effect of Doxorubicin Treatment on the Differential Expression of lncRNAs in Canine Mammary Tumor CHMp Cell Line

作　　者：张琰 吴梅金 周家豪 刁洪秀 ZHANG Yan;WU Meijin;ZHOU Jiahao;DIAO Hongxiu(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province,Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福建省畜禽病原感染与免疫学重点实验室,福州福建350002

出　　处：《畜牧兽医学报》2024年第6期2716-2726,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金青年基金项目(32302946);福建省教育厅中青年教师教育科研项目(JAT210085);新瑞鹏宠物医疗集团有限公司、深圳市瑞鹏公益基金会(KH210163A)。

摘　　要：旨在探究阿霉素对犬乳腺肿瘤细胞中lncRNAs分子的影响,寻找犬乳腺肿瘤潜在生物标志物和治疗靶点,本试验采用RNA-Seq技术对经阿霉素处理的犬乳腺肿瘤细胞进行转录组测序分析,筛选差异表达lncRNAs,应用GO富集和KEGG富集分析进行基因功能和途径注释并验证其表达情况。结果显示,经阿霉素处理后的犬乳腺肿瘤细胞中共有277个差异表达的lncRNAs,其中,147个表达上调,130个表达下调,主要涉及细胞新陈代谢、转录程序失调、ECM受体相互作用以及细胞坏死性凋亡等生物学过程。实时荧光定量PCR技术随机检测3个差异表达的lncRNAs,其表达趋势与RNA-Seq一致,表明RNA-Seq测序结果的准确性。本研究基于转录组学技术,综合分析阿霉素抗犬乳腺肿瘤细胞相关lncRNAs表达变化的整体特征,揭示其可能的调控新机制,为犬乳腺肿瘤的靶向治疗和潜在生物标志物的开发利用提供参考。To explore the effect of doxorubicin on lncRNAs in canine breast tumor cells and find potential biomarkers and therapeutic targets for canine mammary tumor,RNA-Seq technology was used to perform transcriptome sequencing analysis on canine breast tumor cells treated with doxorubicin,and differentially expressed lncRNAs were screened.GO enrichment and KEGG enrichment analysis were used to annotate gene functions and pathways and verify their expression.The results showed that there were 277 differentially expressed lncRNAs in canine breast tumor cells after doxorubicin treatment,of which 147 were up-regulated and 130 were down-regulated,mainly involving biological processes such as cell metabolism,transcriptional program disorder,ECM receptor interaction and cell necroptosis.Three differentially expressed lncRNAs were randomly detected by real-time quantitative PCR,and their expression trends were consistent with RNA-Seq,indicating the accuracy of the RNA-Seq sequencing results.Based on transcriptomics technology,this study comprehensively analyzed the overall characteristics of the expression changes of lncRNAs related to doxorubicin against canine breast tumor cells,revealed its possible new regulatory mechanisms,and provided a reference for the targeted therapy of canine mammary tumor and the development and utilization of potential biomarkers.

关 键 词：lncRNA 犬 乳腺肿瘤细胞 阿霉素 RNA-SEQ 

分 类 号：S857.26[农业科学—临床兽医学]