TRIB3促进喉癌细胞增殖和侵袭迁移的机制研究  

TRIB3 promotes the proliferation,invasion and migration of laryngeal cancer cells

作　　者：冯旭 程忠强[1] 蒋成义[1] Feng Xu;Cheng Zhongqiang;Jiang Chengyi(Department of Otorhinolaryngology Head and Neck Surgery,First Affiliated Hospital of Bengbu Medical Univercity,Bengbu,Anhui,233003,China)

机构地区：[1]蚌埠医学院第一附属医院,安徽蚌埠233003

出　　处：《中国医学文摘（耳鼻咽喉科学）》2024年第3期103-106,共4页Chinese Medical Digest(Otorhinolaryngology)

基　　金：蚌埠医学院自然科学重点课题(项目编号:2021byzd070)安徽省教育厅重点课题(项目编号:2022AH051501)。

摘　　要：目的探讨TRIB3对喉癌细胞增殖和侵袭迁移的影响,并研究其作用机制。方法western blot及免疫组化检测TRIB3在喉癌组织及癌旁组织中的表达情况,并检测正常喉上皮细胞及喉癌细胞株(TU686细胞)中TRIB3的mRNA及蛋白表达情况。下调TRIB3后,通过EDU实验、划痕实验及Transwell实验分析TRBI3对喉癌细胞增殖、迁移及侵袭能力的影响;通过Western Blot检测下调喉癌细胞TRIB3表达后,β-catenin、Dvl2、GSK-3β和p-GSK-3β的表达水平的表达情况。结果喉癌组织TRIB3的表达量显著高于癌旁组织;TRIB3在癌组织表达阳性率显著高于癌旁组织;与正常喉上皮细胞相比,TU686细胞的TRIB3的mRNA及蛋白表达量显著升高;下调TRIB3后,TU686细胞的增殖能力及侵袭迁移能力均显著降低。下调TRIB3后,TU686细胞中Dvl2、p-GSK-3β和β-catenin蛋白的表达水平显著降低,GSK-3β蛋白的表达水平显著升高(P<0.05)。结论TRIB3可以通过激活β-catenin信号通路促进喉癌细胞的增殖和侵袭迁移,提示TRIB3可能是喉癌治疗的潜在靶点。Objective To investigate the effect of TRIB3 on the proliferation,invasion and migration of laryngeal cancer cells,and to study its mechanism.Methods Expression of TRIB3 in laryngeal carcinoma and paracancerous tissues detected by westernblot and immunohistochemistry,and the mRNA and protein expression of TRIB3 in normal laryngeal epithelial cells and laryngeal cancer cell lines(TU686 cells)were detected.After downregulation of TRIB3,the effects of TRBI3 on the proliferation,migration and invasion of laryngeal cancer cells were analyzed by EDU test,scratch test and Transwell test,and the expression levels ofβ-catenin,Dvl2,GSK-3βand p-GSK-3βwere detected by WesternBlot.Results The expression of TRIB3 in laryngeal carcinoma was significantly higher than that in paracancerous tissues;The positive rate of TRIB3 expression in cancer tissues was significantly higher than that in paracancerous tissues;compared with normal laryngeal epithelial cells,the mRNA and protein expression of TRIB3 in TU686 cells increased significantly;after down-regulation of TRIB3,the proliferation,invasion and migration ability of TU686 cells were significantly decreased.After down-regulation of TRIB3,the expression of Dvl2,p-GSK-3βandβ-catenin protein in TU686 cells decreased significantly,while the expression level of GSK-3βprotein increased significantly.Conclusion TRIB3 can promote the proliferation,invasion and migration of laryngeal cancer cells by activatingβ-catenin signaling pathway,suggesting that TRIB3 may be a potential target for the treatment of laryngeal cancer.

关 键 词：TRIB3 喉癌 增殖 Β-CATENIN 

分 类 号：R73[医药卫生—肿瘤]