响应面法优化黄精膏的水提工艺及体外抗氧化活性的研究  

Optimization of water extraction process and in vitro antioxidant activity of Polygonati rhizoma extracts by Response surface methodology

作　　者：罗霜 王剑波 马佳丽 王成 付家莉 尤晓舟 LUO Shuang;WANG Jianbo;MA Jiali;WANG Cheng;FU Jiali;YOU Xiaozhou(College of Food and Bioengineering,Chengdu University,Chengdu,Sichuan,610106 P.R.China;TCM Key Laboratory of Sichuan Province,Sichuan Institute for TCM,Sichuan Academy of Chinese Medicine Sciences,Chengdu,Sichuan,610041 P.R.China;Junlian County in Sichuan Province Centre for the Development of Chinese Medicine,Yibin,Sichuan,645250 P.R.China)

机构地区：[1]成都大学食品与生物工程学院,四川成都610106 [2]四川省中医药科学院,四川省中医药转化医学中心,中医药转化医学四川省重点实验室,四川成都610041 [3]四川省筠连县中医药发展中心,四川宜宾645250

出　　处：《华西药学杂志》2024年第3期314-320,共7页West China Journal of Pharmaceutical Sciences

基　　金：四川中医药省级发展专项资金资助项目(川中医药办发[2020]39号)。

摘　　要：目的优选黄精膏的水提工艺,研究其体外抗氧化活性。方法采用主客观组合赋权结合响应面法、HPLC法,以料液比、提取时间、提取次数为考察因素,6-姜辣素、总多糖的含量及干浸膏得率为评价指标,优化黄精膏的水提工艺,并评价最佳工艺下黄精膏水提液的体外抗氧化活性。结果黄精膏的最佳水提工艺为料液比1:8,提取次数3次,提取时间1.5 h,该条件下黄精膏水提液的综合评分为44.974,预测结果的平均得分为49.881,相对误差为3.494%,与预测值的得分接近一致;DPPH自由基、羟基自由基清除率随水提液的浓度增加而增大,50 mg·mL^(-1)黄精膏水提液对羟基自由、DPPH自由基的清除率分别为89.49%±0.02%、83.80%±0.01%。结论优选的水提工艺稳定,合理可行,水提液具有较好的抗氧化活性,可为黄精膏药食同源产品的后续开发提供依据。OBJECTIVE To optimize the water extraction process of Polygonati Rhizoma extracts(PRE)and to study its in vitro antioxidant activity.METHODS We utilized the combination of subjective and objective weighting combined with the response surface method and HPLC.Taking material-liquid ratio,extraction time,and extraction times as inspection factors was adopted.We also took 6-gingerol,total polysaccharides content and dry extract yield as the evaluation indicators.We also aimed at optimizing the water extraction process of PRE,and in vitro antioxidant activity evaluation of PRE under the best technology.RESULTS The results showed that the optimal water extraction process of PRE was I:8 with the ratio of solid to liquid,the extraction times were 3 times,and the extraction time was 1.5 h.The relative error was 3.494%,which was close to the predicted value.The antioxidant activity test showed that the removal rate of DPPH free radicals and hydroxyl radicals increased with the mass concentration of the aqueous extract.At 50 mg·mL^(-1),the removal rate of hydroxyl free radicals and DPPH free radicals in the aqueous extract was 89.49%±0.02%and 83.80%±0.01%,respectively.CONCLUSION The optimized water extraction process is stable,reasonable and feasible,and the water extraction solution has the good antioxidant activity,which can provide the basis for the further development of PRE ointment food homologous products.

关 键 词：黄精膏 响应面法 水提工艺 6-姜辣素 总多糖 抗氧化活性 高效液相色谱法 药食同源 

分 类 号：R917[医药卫生—药物分析学]