佳木斯市肠炎沙门菌的耐药特征及同源性分析  

Evolutionary associations of drug resistance in Salmonella enteritidis in Jiamusi City

作　　者：付宇[1] 吴晓敏[1] 赵婧 孟庆敏[1] 孟敏芝[1] 王彬[1] 郝芸 包名家[1] FU Yu;WU Xiaomin;ZHAO Jing;MENG Qingmin;MENG Minzhi;WANG Bin;HAO Yun;BAO Mingjia(Jiamusi Center for Disease Control and Prevention;The First Affiliated Hospital of Jiamusi University;School of Public Health,Jiamusi University)

机构地区：[1]佳木斯市疾病预防控制中心,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院,黑龙江佳木斯154007 [3]佳木斯大学公共卫生学院,黑龙江佳木斯154007

出　　处：《中国食品卫生杂志》2024年第3期314-319,共6页Chinese Journal of Food Hygiene

基　　金：佳木斯市市级科技计划创新激励类项目(SF2023JL0013)。

摘　　要：目的了解佳木斯市优势血清型肠炎沙门菌的耐药状况及进化关系,为临床治疗提供科学依据。方法对来自食品和腹泻患者的沙门菌进行血清学分型,采用微量肉汤稀释法对分离的肠炎沙门菌进行16种抗生素药敏试验,脉冲场凝胶电泳分析菌株之间的进化关系。结果在108株沙门菌中,检出肠炎沙门菌55株(50.93%);49株肠炎沙门菌有耐药性,耐药率为89.09%,其中33株菌(67.35%)为多重耐药,对抗生素萘啶酸和氨苄西林的耐药率较高,分别为81.82%和60.00%;55株肠炎沙门菌同源性为37%~100%。结论佳木斯地区肠炎沙门菌多重耐药率高,菌株之间亲缘关系和耐药性存在密切关联性,来自食品和腹泻患者的肠炎沙门菌有交叉感染现象,依据本地区肠炎沙门菌耐药特征,制定有针对性诊疗方案和预防控制措施,对保障公共卫生健康发展具有重要意义。Objective The study investigated the evolutionary associations of drug resistance in main serotype Salmonella enteritidis(S.enteritidis)in Jiamusi City,to provide a scientific basis for clinical treatment.Methods S.enteritidis was isolated from food and patients with diarrhea.Serological typing was performed.Resistance against 16antibiotics was investigated using the microbroth dilution method.Genes associated with antimicrobial resistance were analyzed by PCR,and the evolutionary relationships between strains were analyzed by pulsed-field gel electrophoresis(PFGE).Results Of the 108 Salmonella strains identified,55(50.93%)were found to be S.enteritidis.Forty-nine(89.09%)of these strains showed antimicrobial resistance,with 33 strains(67.35%,33/49)found to be resistant to multiple antibiotics.The highest resistance was against nalidixic acid and ampicillin,with 81.82%and 60.00%resistance,respectively.Homologies between 37%and 100%were observed among the 55 S.enteritidis strains.Conclusion High levels of multi-antimicrobial resistance were found in S.enteritidis in Jiamusi.A close relationship was found between,pathogen strain and drug resistance.S.enteritidis in the diarrhea of infected patients can cross-infect food.As S.enteritidis shows significant drug resistance in this region,it is of great importance to develop targeted diagnostic and treatment programs,as well as prevention and control measures,to ensure the safety and maintenance of public health.

关 键 词：肠炎沙门菌 耐药性 同源性 进化 

分 类 号：R155[医药卫生—营养与食品卫生学]