菊睛方对碘酸钠诱导的干性年龄相关性黄斑变性小鼠视网膜的保护作用及物质基础研究  

The protective effect of Jujing formula on tretina of mice with dry age-related macular degeneration

作　　者：齐程成 范若颖 王学森 宿树兰[1] 朱悦[1] 郭盛[1] 康宏杰 周学义 段金廒[1] QI Cheng-cheng;FAN Ruo-ying;WANG Xue-sen;SU Shu-lan;ZHU Yue;GUO Sheng;KANG Hong-jie;ZHOU Xue-yi;DUAN Jin-ao(Nanjing University of Chinese Medicine/National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Jiangsu Province Key Laboratory of High Technology Research,Nanjing 210023,China;Ningxia Innovation Center of Goji,Yinchuan 750000,China;Ningxia Love GOJI Supply China Corporation,Zhongning Ningxia 755199,China)

机构地区：[1]南京中医药大学/中药资源产业化与方剂创新药物国家地方联合工程中心,江苏省中药资源产业化过程协同创新中心,江苏省方剂高技术研究重点实验室,江苏南京210023 [2]宁夏枸杞创新中心,宁夏银川750000 [3]宁夏全通枸杞供应链管理股份有限公司,宁夏中宁755199

出　　处：《中国药理学通报》2024年第7期1358-1367,共10页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金区域创新发展联合基金重点项目(No U21A20408);宁夏重点研发计划重点项目(No 2021BEF02009,2021BEF02010,2021BEF02012,2022BFH02008);江苏省高等学校基础科学(自然科学)研究重大项目(No 22KJA360008);江苏省中药资源产业化过程协同创新中心重点项目(No ZDXM-2020-03);江苏省研究生科研与实践创新计划(No KYCX23_2018)。

摘　　要：目的研究菊睛方对干性年龄相关性黄斑变性(age-related macular degeneration,AMD)小鼠视网膜的保护作用。方法采用腹腔注射碘酸钠的方法诱导小鼠干性AMD模型,给予菊睛方干预后,运用光学相干断层扫描(OCT)检查视网膜厚度,苏木精-伊红(HE)染色观察视网膜形态学变化,原位末端转移酶标记(TUNEL)染色检测视网膜细胞凋亡情况,结合眼球及血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α),白介素-6(interleukin-6,IL-6)和白介素-1β(interleukin-1β,IL-1β)炎症因子以及超氧化物歧化酶(superoxide dismutase,SOD),谷胱甘肽(glutathione,GSH)及丙二醛(malondialdehyde,MDA)含量,评价菊睛方对干性AMD小鼠的视网膜保护作用。结果OCT、HE及TUNEL染色结果显示,菊睛方可明显改善碘酸钠诱导的干性AMD小鼠视网膜损伤,增加视网膜厚度(P<0.05),减少视网膜细胞的凋亡(P<0.01),提高眼球及血清中GSH和SOD活性(P<0.01),降低TNF-α、IL-6、IL-1β及MDA水平(P<0.01)。结论菊睛方能有效改善干性AMD小鼠视网膜损伤,其作用途径与抑制炎性反应、增强抗氧化能力密切相关。Aim To investigate the protective effect of Jujing formula on retina of mice with dry age-related macular degeneration(AMD).Methods The mouse model of dry AMD was induced by intraperitoneal injection of sodium iodate,and the prognosis was given to the Jujing formula.Retinal thickness was detected by optical coherence tomography(OCT),the retinal morphological changes were observed by hematoxylin-eosin(HE)staining,and the apoptosis of retinal cells was detected by in situ terminal transferase labeling(TUNEL)staining.Combination of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in eyeballs and serum,superoxide dismutase(SOD),glutathione(GSH)and malondialdehyde(MDA)were evaluated to assess the protective effects of Jujing formula on retinal injury in mice with dry AMD.Results The results of OCT,HE and TUNEL staining showed that Jujing formula significantly improved the retinal injury induced by sodium iodate in mice with dry AMD,increased the retinal thickness(P<0.05),reduced the apoptosis of retinal cells(P<0.01),and increased the levels of GSH,IL-6 and SOD activity in eyeballs and serum(P<0.01).The levels of TNF-α,IL-6,IL-1βand MDA were reduced(P<0.01).Conclusions Jujing formula has certain therapeutic effects on retinal injury in dry AMD,which may be related to inhibiting inflammatory response and enhancing antioxidant capacity.

关 键 词：菊睛方 干性年龄相关性黄斑变性 视网膜损伤 抗氧化 炎症因子 凋亡 

分 类 号：R-332[医药卫生] R289.5R322.91R329.25R364.5R774.5