miR-125a对子宫内膜癌细胞生物学行为的影响及机制  

Effects and mechanism of miR-125a on biological behaviors of endometrial cancer cells

作　　者：何建清 杨立芬 殷悦 陈莹[1] HE Jianqing;YANG Lifen;YIN Yue;CHEN Ying(Department of Gynaecology and Obstetrics,Tangshan Maternal and Child Health Hospital,Tangshan 063000,China)

机构地区：[1]唐山市妇幼保健院妇产科,河北唐山063000

出　　处：《山东医药》2024年第19期45-48,共4页Shandong Medical Journal

基　　金：河北省医学科学研究课题计划项目(20211490)。

摘　　要：目的探讨微小RNA-125a(miR-125a)对子宫内膜癌(EC)细胞增殖、凋亡、迁移及侵袭等生物学行为的影响及机制。方法常规培养人EC细胞(JEC、HEC-1B、Ishikawa细胞)和子宫内膜上皮细胞(ECS细胞)。用RT-PCR法检测细胞中miR-125a表达,筛选miR-125a表达最低的细胞为实验细胞。取对数生长期miR-125a表达最低的EC细胞,并将其随机分为miR-NC组、miR-125a mimics组,分别转染miRNA阴性对照miR-NC、miR-125a模拟物miR-125a mimics。用RT-PCR法检测miR-125a表达,用CCK-8实验检测细胞增殖活性(OD值),用平板克隆实验检测细胞集落形成能力,用流式细胞术检测细胞凋亡能力(凋亡率),用Transwell实验检测细胞迁移、侵袭能力,Western blotting法检测细胞内LIN28同系物B(LIN28B)蛋白表达。结果与ECS细胞比较,JEC、HEC-1B、Ishikawa细胞中miR-125a相对表达量均低(P均<0.05),且Ishikawa细胞miR-125a的相对表达量最低,将其作为实验细胞。与miR-NC组比较,miR-125a mimics组miR-125a表达高(P<0.05)。与miR-NC组比较,miR-125a mimics组培养24、48、72 h后OD值低,集落形成数少,凋亡率高,迁移细胞数、侵袭细胞数少,LIN28B蛋白相对表达量低,比较差异均有统计学意义(P均<0.05)。结论上调miR-125a表达可抑制EC细胞增殖、迁移及侵袭,并诱导其凋亡,该作用可能与其靶向调控LIN28B表达有关。Objective To investigate the effects of microRNA-125a(miR-125a)on the proliferation,apoptosis,migration,invasion,and other biological behaviors of endometrial cancer(EC)cells and the mechanism.Methods Human EC cells(JEC,HEC-1B,and Ishikawa cells)and endometrial epithelial cells(ECS cells)were cultured routinely.The expression of miR-125a in cells was detected by RT-PCR,and the cells with the lowest expression of miR-125a were screened as experimental cells.EC cells in the logarithmic growth phase and with the lowest expression of miR-125a were taken and randomly divided into miR-NC group and miR-125a mimics group,which were transfected with miRNA negative control miR-NC and miR-125a mimics,respectively.The expression of miR-125a was detected by RT-PCR,the cell proliferation activity(OD value)was detected by CCK-8 assay,the cell colony formation ability was detected by plate cloning assay,the apoptosis ability(apoptosis rate)was detected by flow cytometry,the cell migration and invasion capacities were detected by Transwell chamber assay,and the intracellular LIN28B protein expression was detected by Western blotting.Results Compared with ECS cells,the relative expression levels of miR-125a in JEC,HEC-1B and Ishikawa cells were lower(all P<0.05),and the relative expression of miR-125a in Ishikawa cells was the lowest,which were used as experimental cells.Compared with the miR-NC group,the expression of miR-125a in the miR-125a mimics group was higher(P<0.05).Compared with the miR-NC group,the OD values of miR-125a mimics group were lower after culture for 24,48,and 72 h,and the number of colony formation was smaller,the apoptosis rate was higher,the migrating cells and invasive cells were less,and the relative expression of LIN28B protein was lower,and the differences were statistically significant(all P<0.05).Conclusion Up-regulation of miR-125a expression inhibits the proliferation,migration and invasion of EC cells and induces apoptosis,which may be related to its targeted regulation of LIN28B gene.

关 键 词：子宫内膜癌 微小核糖核酸-125a LIN28同系物B 细胞增殖 细胞凋亡 细胞迁移 细胞侵袭 

分 类 号：R737.33[医药卫生—肿瘤]