肝癌中PP2A B56家族成员对于预后的价值以及免疫浸润分析  

The prognostic value of PP2A B56 family members in hepatocellular carcinoma and immune infiltration analysis

作　　者：向未来 吴红梅 黄媛媛 许力 李迪 朱小年 XIANG Weilai;WU Hongmei;HUANG Yuanyuan;XU Li;LI Di;ZHU Xiaonian(School of Public Health,Guilin Medical University,Guilin 541199,Guangxi,China;Guangxi Key Laboratory of Environmental Exposure Omics and Life Cycle Health,Guilin 541199,Guangxi,China)

机构地区：[1]桂林医学院公共卫生学院,广西桂林541199 [2]广西环境暴露组学与全生命周期健康重点实验室,广西桂林541199

出　　处：《右江民族医学院学报》2024年第3期320-327,共8页Journal of Youjiang Medical University for Nationalities

基　　金：广西重点研发计划项目(桂科AB22035017)。

摘　　要：目的探讨肝癌中蛋白磷酸酶2A(PP2A)B56家族成员对于预后的价值。方法利用GEPIA、Kaplan-Meier绘图仪、cBioPortal、GeneMANIA和TIMER分析肝癌患者PP2A B56的差异表达、预后价值、基因改变和免疫细胞浸润。结果肝癌组织中PPP2R5A、PPP2R5C、PPP2R5D和PPP2R5E的表达水平升高,而PPP2R5E与肝癌患者的临床癌症分期和总生存期显著相关,PPP2R5B、PPP2R5D与肝癌患者的总生存期或者无病生存期显著相关,提示PPP2R5D、PPP2R5E可能是肝癌患者生存的潜在预后生物标志物。此外,差异表达的PP2A B56家族成员的功能主要是与其他如SGO1、PFKFB4、PPP2R1A、NKD1等蛋白分子参与的糖酵解、蛋白质丝氨酸/苏氨酸磷酸复合物、蛋白质去磷酸化、减数分裂、核苷酸代谢、JAK-STAT信号通路等有关。进一步分析发现PP2A B56的表达与肝癌中6种免疫细胞,包括B细胞、CD8^(+)T细胞、CD4^(+)T细胞、巨噬细胞、中性粒细胞和树突状细胞的免疫浸润显著相关。结论PPP2R5D、PPP2R5E可作为肝癌潜在的预后生物标志物。Objective To investigate the prognostic value of protein phosphatase 2A(PP2A)B56 family members in hepatocellular carcinoma(HCC).Methods The GEPIA,Kaplan-Meier plotter,cBioPortal,GeneMANIA and TIMER databases were used to analyze the differential expression,prognostic value,genetic alterations,and immune cell infiltration of PP2A B56 family members in HCC patients.Results The expression levels of PPP2R5A,PPP2R5C,PPP2R5D,and PPP2R5E were elevated in HCC tissues.PPP2R5E exhibited a significant correlation with clinical cancer stage and overall survival,and PPP2R5B and PPP2R5D were significantly associated with overall survival or disease-free survival in HCC patients.These findings suggest that PPP2R5D and PPP2R5E may serve as potential prognostic biomarkers for HCC patients.Furthermore,the differentially expressed PP2A B56 family members were functionally involved in glycolysis,protein serine/threonine phosphate complexes,protein dephosphorylation,meiosis,nucleotide metabolism,and the JAK-STAT signaling pathway through interactions with proteins such as SGO1,PFKFB4,PPP2R1A,and NKD1.Additionally,the expression of PP2A B56 members demonstrated a significant correlation with the immune infiltration of B cells,CD8^(+)T cells,CD4^(+)T cells,macrophages,neutrophils and dendritic cells in HCC.Conclusion PPP2R5D and PPP2R5E may serve as potential prognostic biomarkers for HCC.

关 键 词：生物信息学分析 PP2A B56家族 癌 肝细胞 生物标志物 

分 类 号：R730.261[医药卫生—肿瘤]