A Label-Free Deoxyribozyme Sensor for m^(6)A Demethylase Activity Detection  

作　　者：Xiuyuan Wang Shusheng Yu Yuqiu He Fuan Wang Xiaoqing Liu 

机构地区：[1]College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,Hubei 430072,China

出　　处：《Chinese Journal of Chemistry》2024年第9期951-956,共6页中国化学（英文版）

基　　金：supported by the Natural Science Foundation of Jiangsu Province(BK20221258);the National Natural Science Foundation of China(22274121 and 22274123).We thank Prof.Xiang Zhou at Wuhan University for CD measurement.

摘　　要：A label-free deoxyribozyme(DNAzyme)biosensor for m^(6) demethylase activity detection is developed.When demethylase FTO(Fat mass and obesity-associated protein),an important m^(6) demethylase of ALKBH demethylase family is present,the m^(6) group is specifically demethylated,and the cleavage activity of DNAzyme is restored.This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T(ThT)and generate fluorescence signals.The biosensor shows high specificity and sensitivity,and fast reaction speed.Our study demonstrates a new design of allosteric DNAzyme for sensing.This method represents the first label-free nucleic acid biosensor for FTO assay,providing a feasible route towards inhibitors screening.

关 键 词：DNAZYME Analytical methods BIOSENSING m^(6)A demethylation Fluorescence 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]