Effects of antioxidant‑rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats  

作　　者：Yixin Zhang Samaila Usman Qiang Li Fuhou Li Xia Zhang Luiz Gustavo Nussio Xusheng Guo 

机构地区：[1]State Key Laboratory of Grassland Agro‑ecosystems,School of Life Sciences,Lanzhou University,Lanzhou 730000,PR China [2]Probiotics and Life Health Institute,Lanzhou University,Lanzhou 730000,PR China [3]Probiotics and Biological Feed Research Centre,Lanzhou University,Lanzhou 730000,PR China [4]Department of Animal Science,Luiz de Queiroz College of Agriculture,University of Sao Paulo,Piracicaba 13418‑900,Brazil

出　　处：《Journal of Animal Science and Biotechnology》2024年第3期1227-1240,共14页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. U20A2002);China Postdoctoral Science Foundation (No. 2023T160284);recipient of a research productivity fellowship from CNPq (National Council of Scientific and Technological Development) in Brazil

摘　　要：Background Milk synthesis in lactating animals demands high energy metabolism,which results in an increased production of reactive oxygen metabolites(ROM)causing an imbalance between oxidants and antioxidants thereby inducing oxidative stress(OS)on the animals.To mitigate OS and postpartum disorders in dairy goats and gain insight into the impact of dietary choices on redox status during lactation,a feeding trial was conducted using alfalfa silage inoculated with a high-antioxidant strain of Lactiplantibacillus plantarum.Methods Twenty-four Guanzhong dairy goats(38.1±1.20 kg)were randomly assigned to two dietary treatments:one containing silage inoculated with L.plantarum MTD/1(RSMTD-1),and the other containing silage inoculated with high antioxidant activity L.plantarum 24-7(ES24-7).Results ES24-7-inoculated silage exhibited better fermentation quality and antioxidant activity compared to RSMTD-1.The ES24-7 diet elevated the total antioxidant capacity(T-AOC),superoxide dismutase(SOD),glutathione peroxi-dase(GSH-Px),and catalase(CAT)activities in milk,serum,and feces of lactating goats(with the exception of T-AOC in milk).Additionally,the diet containing ES24-7 inoculated silage enhanced casein yield,milk free fatty acid(FFA)content,and vitamin A level in the goats’milk.Furthermore,an increase of immunoglobulin(Ig)A,IgG,IgM,inter-leukin(IL)-4,and IL-10 concentrations were observed,coupled with a reduction in IL-1β,IL-2,IL-6,interferon(IFN)-γ,and tumor necrosis factor(TNF)-αconcentrations in the serum of lactating goats fed ES24-7.Higher concentrations of total volatile fatty acid(VFA),acetate,and propionate were observed in the rumen fluid of dairy goats fed ES24-7 inoculated silage.Moreover,the diet containing ES24-7 inoculated silage significantly upregulated the expression of nuclear factor erythroid 2 like 2(NFE2L2),beta-carotene oxygenase 1(BCO1),SOD1,SOD2,SOD3,GPX2,CAT,glu-tathione-disulfide reductase(GSR),and heme oxygenase 1(HMOX1)genes in the mammary gland,while decreased the levels of NADPH oxidas

关 键 词：Alfalfa silage Antioxidant activity Gene expression Goats IMMUNITY LACTATION 

分 类 号：S827.5[农业科学—畜牧学]