Exploring the modulatory role of bovine lactoferrin on the microbiome and the immune response in healthy and Shiga toxin‑producing E.coli challenged weaned piglets  

作　　者：Matthias Dierick Ruben Ongena Daisy Vanrompay Bert Devriendt Eric Cox 

机构地区：[1]Laboratory of Immunology,Department of Translational Physiology,Infectiology and Public Health,Faculty of Veterinary Medicine,Ghent University,Salisburylaan 133,9820 Merelbeke,Belgium [2]Laboratory for Immunology and Animal Biotechnology,Faculty of Bioscience Engineering,Ghent University,Coupure links 653,9000 Ghent,Belgium

出　　处：《Journal of Animal Science and Biotechnology》2024年第3期1278-1291,共14页畜牧与生物技术杂志（英文版）

基　　金：The research that yielded these results,was funded by the Belgian Federal Public Service of Health,Food Chain Safety and Environment through the contract RF 17/6314 LactoPigHealth;Matthias Dierick is supported by the Flemish fund for scientific research(FWO;3S036319).

摘　　要：Background Post-weaned piglets suffer from F18+Escherichia coli(E.coli)infections resulting in post-weaning diar-rhoea or oedema disease.Frequently used management strategies,including colistin and zinc oxide,have contrib-uted to the emergence and spread of antimicrobial resistance.Novel antimicrobials capable of directly interacting with pathogens and modulating the host immune responses are being investigated.Lactoferrin has shown promising results against porcine enterotoxigenic E.coli strains,both in vitro and in vivo.Results We investigated the influence of bovine lactoferrin(bLF)on the microbiome of healthy and infected weaned piglets.Additionally,we assessed whether bLF influenced the immune responses upon Shiga toxin-producing E.coli(STEC)infection.Therefore,2 in vivo trials were conducted:a microbiome trial and a challenge infection trial,using an F18+STEC strain.BLF did not affect theα-andβ-diversity.However,bLF groups showed a higher relative abundance(RA)for the Actinobacteria phylum and the Bifidobacterium genus in the ileal mucosa.When analysing the immune response upon infection,the STEC group exhibited a significant increase in F18-specific IgG serum levels,whereas this response was absent in the bLF group.Conclusion Taken together,the oral administration of bLF did not have a notable impact on theα-andβ-diversity of the gut microbiome in weaned piglets.Nevertheless,it did increase the RA of the Actinobacteria phylum and Bifi-dobacterium genus,which have previously been shown to play an important role in maintaining gut homeostasis.Furthermore,bLF administration during STEC infection resulted in the absence of F18-specific serum IgG responses.

关 键 词：E.COLI Immune modulation LACTOFERRIN MICROBIOME 

分 类 号：S828.5[农业科学—畜牧学]