重组烟酰胺核糖激酶催化合成烟酰胺单核苷酸  

Synthesis of Nicotinamide Mononucleotide Catalyzed by Recombinant Nicotinamide Ribokinase

作　　者：张佳丽 吴英捷 董文兴 徐伟杰 周爱 尹龙飞[1] ZHANG Jia-li;WU Ying-jie;DONG Wen-xing;XU Wei-jie;ZHOU Ai;YIN Long-fei(School of Life Science,Taizhou University,Zhejiang Taizhou 318000,China)

机构地区：[1]台州学院生命科学学院,浙江台州318000

出　　处：《广州化工》2024年第5期46-49,共4页GuangZhou Chemical Industry

基　　金：国家级大学生创新创业训练计划项目(202110350017);浙江省大学生科技创新活动计划(新苗人才计划)项目(2021R436007)。

摘　　要：烟酰胺单核苷酸广泛应用在食品、制药、美容等领域。它是NAD+的关键前体之一,其具有很大的抗衰老的潜力,受到科学界和工业界的广泛关注。本研究首先构建出表达烟酰胺核糖激酶的重组E.coli BL21(DE3)/pET28b-NRK,然后优化蛋白表达条件,再通过单因素实验优化酶催化反应条件。结果表明最佳催化反应条件为:30℃,pH 7.0,1 g/L粗酶液,6 g/L烟酰胺核糖,6 g/L ATP,4 g/L MgSO_(4),在10 mL的反应体系中振荡反应30 min,产物NMN可达6.384 g/L,收率为97.9%。本研究为酶法合成NMN的应用研究奠定了基础。Nicotinamide mononucleotide was widely used in food,pharmaceutical,beauty and other fields.It was one of the key precursors of NAD^(+),which has great anti-aging potential,and has been widely concerned by the scientific and industrial communities.Recombinant E.coli BL21(DE3)/pET28b-NRK expressing nicotinamide ribokinase was constructed,and then the expression conditions of the protein were optimized,and the enzymatic reaction conditions were optimized by single factor experiment.The results showed that the optimal catalytic reaction conditions were as follows:at 30℃,pH 7.0,1 g/L crude enzyme solution,6 g/L nicotinamide ribose,6 g/L ATP,4 g/L MgSO_(4),the product NMN reached 6.384 g/L and the yield was 97.9% after incubation for 30 min in a 10 mL reaction system.This study laid a foundation for the application of enzymatic synthesis of NMN.

关 键 词：烟酰胺单核苷酸 烟酰胺核糖 NAD^(+) 生物合成 化学合成 

分 类 号：R914[医药卫生—药物化学]