基于灰色关联度分析砂仁传统性状鉴别特征与内在成分含量的相关性研究  

Correlation between morphological traits of Amomi Fructus and contents of internal components based on grey relational analysis

作　　者：崔阳 米文娟 李阳[1] 韩玉[1] 翟恩爱 王亚顺 邹慧琴[1] 闫永红[1] CUI Yang;MI Wenjuan;LI Yang;HAN Yu;ZHAI Enai;WANG Yashun;ZOU Huiqin;YAN Yonghong(Beijing University of Chinese Medicine,Beijing 100029,China;NanoRibo(Shanghai)Biotechnology Co.,Ltd.,Shanghai 201112,China)

机构地区：[1]北京中医药大学,北京100029 [2]纳美信(上海)生物科技有限公司,上海201112

出　　处：《中华中医药杂志》2024年第6期3036-3043,共8页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81403054)。

摘　　要：目的:以砂仁中的非挥发性成分作为目标成分,与目前公认的挥发油成分进行比较,分析其与传统性状的相关性,以此验证所筛选的砂仁质量评价指标的合理性与科学性。方法:以砂仁作为研究对象,采用HPLC测定砂仁中的非挥发性成分(槲皮苷、C1、C17)的含量,GC测定砂仁中主要的4个挥发性成分(龙脑、樟脑、柠檬烯、乙酸龙脑酯)的含量;通过电子鼻及百果重、横径、纵径、纵径/横径比的测定,对砂仁传统性状鉴别特征进行量化分析,基于灰色关联度分析将两类化学成分与性状指标进行相关性研究。结果:由灰色关联分析结果可知,关联度由大到小为:龙脑(r=0.751)>槲皮苷(r=0.745)>樟脑(r=0.711)>柠檬烯(r=0.682)>C1(r=0.568)>乙酸龙脑酯(r=0.540)>C17(r=0.505),槲皮苷作为非挥发性成分与性状的关联程度仅次于龙脑,可作为补充成分以完善现有的砂仁质量标准。结论:通过相关性分析不仅可阐明砂仁传统性状鉴别特点的科学性,体现了中药“辨状论质”的整体思想,同时可将目标成分与药材质量有效结合,有利于砂仁质量评价体系的完善。Objective:The non-volatile components in Amomi Fructus were used as the target components,which were further compared with the currently recognized volatile oil components,and then the correlation between those two groups of key morphologic traits of Amomi Fructus was analyzed,so as to illustrate the rationality and scientificity of the quality evaluation indicators of Amomi Fructus.Methods:Taking different batches of Amomi Fructus samples from different varieties as the research objects,firstly HPLC was used to determine the content of non-volatile components(quercetin,C1,C17),whilst GC to determine the content of four main volatile components(borneol,camphor,limonene,and bornyl acetate) in Amomi Fructus.Secondly,through the electronic nose and the measurement of 100-fruit weight,transverse diameter,longitudinal diameter,and longitudinal diameter/transverse diameter ratio,a quantitative analysis was conducted on the traditional character identification characteristics of Amomi Fructus.Thirdly,grey correlation analysis was employed to analyze the correlation between inner chemical compounds and external morphological traits.Results:The results show that the correlation degree is as follows,from high to low:borneol(r=0.751)>quercitrin(r=0.745)>camphor(r=0.711)>limonene(r=0.682)>C1(r=0.568)>borneol acetate(r=0.540)>C17(r=0.505).Obviously,quercitrin as a non-volatile monitoring component,its degree of association with traits was second only to borneol,it was suggested that quercetin should be considered as a supplement to complement existing Amomi Fructus quality standards.Conclusion:The correlation analysis could not only clarify the scientificity of the traditional character identification characteristics of Amomi Fructus,reflects the overall idea of ‘assessing inner quality by distinguishing external features based on human organoleptic experience',but also effectively combine the target components with the quality of the medicinal materials,which is beneficial to the improvement of the Amomi Fructus quality ev

关 键 词：砂仁 性状特征 挥发性成分 非挥发性成分 相关性 质控指标 槲皮苷 

分 类 号：R284.1[医药卫生—中药学]