中华猕猴桃AcNOD基因的生物信息学分析  

Bioinformatics Analysis of AcNOD Gene from A ctinidia chinensis

作　　者：王素杰 杨彦萍 师万源 赵文娟 员楠 张汉尧[1] Wang Sujie;Yang Yanping;Shi Wanyuan;Zhao Wenjuan;Yun Nan;Zhang Hanyao(Key Laboratory of Biodiversity Conservation in Southwest China(State Forest Administration),Southwest Forestry University,Kunming,650224;Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China(Ministry of Education),Southwest Forestry University,Kunming,650224)

机构地区：[1]西南林业大学,国家林业局西南地区生物多样性保育重点实验室,昆明650224 [2]西南林业大学,西南山地森林资源保育与利用教育部重点实验室,昆明650224

出　　处：《分子植物育种》2024年第12期3864-3870,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31760450);云南省农业基础研究联合专项(2018FG001-038)共同资助。

摘　　要：结瘤素(nodulin,NOD)是一类主要存在于根瘤细胞中并参与共生固氮作用的植物基因产物,在豆科植物的其他组织细胞中以及非豆科植物体内都有发现。为了探究NOD基因在中华猕猴桃这类非豆科植物中的作用和功能,本研究对其编码序列进行生物信息学分析。结果显示,该基因编码蛋白的分子式为C_(432)H_(715)N_(131)O_(120)S_(3),含有94个氨基酸残基,等电点pI为10.68,属于疏水蛋白。带正电的残基(Arg+Lys)11个,带负电的残基(Asp+Glu)3个,不稳定系数为14.00,脂肪系数为107.23,总平均亲水性系数为0.395;AcNOD蛋白含有一个ENOD93保守结构域;二级结构显示该蛋白主要由59.57%的α-螺旋、17.02%的无规卷曲、15.96%的延伸链和7.45%的β-转角所组成;三级结构的预测结果显示该蛋白扭曲和折叠较多,亚细胞定位于内质网中,存在4个糖基化位点的12个磷酸化位点,不存在信号肽序列,有两个跨膜区域。进化树分析表明该蛋白与豆科植物结瘤素的同源性很高,初步推测他们有相似或相同的功能。本研究结果为后续深入了解NOD基因在中华猕猴桃乃至其他非豆科植物中的作用机制和生物学功能提供基础资料。Nodulin(NOD)is a kind of plant gene product that mainly exists in nodule cells and participates in symbiotic nitrogen fixation,and is found in other tissues and cells of leguminous plants and non-leguminous plants.To explore the biological function of the NOD gene in non-Leguminosae plants such as Actinidia chinensis,this study used a series of bioinformatics methods to analyze its sequence characteristics.The results showed that the number of amino acid residues of the protein encoded by the gene is 94,the molecular formula of the protein is C_(432)H_(715)N_(131)O_(120)S_(3),the isoelectric point pl of the protein is 10.68,which belongs to hydrophobic protein.There are 11 positively charged residues(Arg+Lys)and 3 negatively charged residues(Asp+Glu),the instability coefficient is 14.00,the fat coefficient is 107.23,and the total average hydrophilic coefficient is 0.395;AcNOD protein contains a conserved domain of ENOD93;the results of the secondary structure prediction showed that the protein is mainly composed of 59.57%α-helix,17.02%random curl,15.96%extended chain and 7.45%β-turn;the results of the tertiary structure prediction showed that the protein is twisted and folded more,subcellular localization is in the endoplasmic reticulum,there are 12 phosphorylation sites of 4 glycosylation sites,no signal peptide sequence,and two transmembrane regions.The results of phylogenetic tree analysis showed that the protein has high homology with leguminous nodulin,and has a similar or identical function.The results provided basic data for further understanding the mechanism of the plant NOD gene and the biological function of the AcNOD gene in Actinidiachinensis.

关 键 词：中华猕猴桃 AcNOD基因 蛋白结构 生物信息学 

分 类 号：S663.4[农业科学—果树学]