Jiaohong pills attenuate neuroinflammation and amyloid-βprotein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway  

作　　者：Hong Zhang Weiyan Cai Lijinchuan Dong Qing Yang Qi Li Qingsen Ran Li Liu Yajie Wang Yujie Li Xiaogang Weng Xiaoxin Zhu Ying Chen 

机构地区：[1]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2024年第3期222-233,共12页动物模型与实验医学（英文）

基　　金：The Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical Sciences,Grant/Award Number:CI2023E001TS02,CI2021A04905 and CI2021B015;Key Technology Research Foundation of the National Institutes for Food and Drug Control,Grant/Award Number:GJJS-2022-7-1;the National Natural Science Foundation of China,Grant/Award Number:82074103。

摘　　要：Background:Jiaohong pills(JHP)consist of Pericarpium Zanthoxyli(PZ)and Radix Rehmanniae,two herbs that have been extensively investigated over many years due to their potential protective effects against cognitive decline and memory impairment.However,the precise mechanisms underlying the beneficial effects remain elusive.Here,research studies were conducted to investigate and validate the therapeutic effects of JHP on Alzheimer's disease.Methods:BV-2 cell inflammation was induced by lipopolysaccharide.AD mice were administered amyloid-β(Aβ).Behavioral experiments were used to evaluate learning and memory ability.The levels of nitric oxide(NO),tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),and interleukin-10(IL-10)were detected using enzymelinked immunosorbent assay(ELISA).The protein expressions of inducible nitric oxide synthase(iNOS)and the phosphorylation level of mitogen-activated protein kinase(MAPK)and nuclear factor kappa-B(NF-κB)were detected using Western blot.Nissl staining was used to detect neuronal degeneration.Results:The results demonstrated that an alcoholic extract of PZ significantly decreased the levels of NO,IL-1β,TNF-α,and iNOS;increased the expression level of IL-10;and significantly decreased the phosphorylation levels of MAPK and NF-κB.These inhibitory effects were further confirmed in the AD mouse model.Meanwhile,JHP improved learning and memory function in AD mice,reduced neuronal damage,and enriched the Nissl bodies in the hippocampus.Moreover,IL-1βand TNF-αin the cortex were significantly downregulated after JHP administration,whereas IL-10showed increased expression.Conclusions:It was found that JHP reduced neuroinflammatory response in AD mice by targeting the MAPK/NF-κB signaling pathway.

关 键 词：amyloid-β(Aβ)protein BV2 NEUROINFLAMMATION Pericarpium Zanthoxyli Radix Rehmanniae 

分 类 号：R285[医药卫生—中药学]