PEDV乳酸菌工程菌株对小鼠的免疫效果研究  

Immune Effect of PEDV Lactobacillus Engineering Strain in Mice

作　　者：安琦[1] 牛彦波[1] 吴皓琼[1] 樊川[1] 曹亚彬[1] 原韬[1] AN Qi;NIU Yanbo;WU Haoqiong;FAN Chuan;CAO Yabin;YUAN Tao(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,Heilongjiang,China)

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150000

出　　处：《农学学报》2024年第6期67-71,共5页Journal of Agriculture

基　　金：黑龙江省科学院青年创新基金杰青项目“重组PEDV乳酸工程菌株构建研究”(CXJQ2021SW02)。

摘　　要：本研究以引起猪流行性腹泻的猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)为研究目标,利用含有PEDV S基因的植物乳杆菌活载体菌株LP1522-PEDS口服免疫小鼠,应用ELISA试验检测免疫小鼠粪便中SIgA含量、血清中IgG、IL-4、IL-2和IFN-γ含量,评价乳酸菌重组菌株LP1522-PEDS对小鼠的免疫效果。结果表明,首次免疫后,商品灭活疫苗组和工程菌株免疫组粪便中的SIgA、血清中的IgG、IL-4、IL-2和IFN-γ含量较对照组显著升高(P<0.05)。在免疫42~56 d,灭活疫苗组和重组菌株组抗体水平均达到最大值,抗体和免疫因子水平依次为:商品灭活疫苗组>LP1522-PEDS组>空载组≈对照组。随后商品灭活疫苗组、LP1522-PEDS组抗体和免疫因子水平逐渐下降。免疫70 d,商品灭活疫苗组抗体水平降幅度相对较小,工程菌株免疫组降幅较大。结果表明,小鼠口服携带PEDV S基因的基因工程植物乳杆菌LP1522-PEDS后能够诱导机体产生PEDV免疫应答。Porcine epidemic diarrhea virus(PEDV),which causes porcine epidemic diarrhea,was used as the research target in this study.The live vector strain LP1522-PEDS of Lactobacillus plantarum containing PEDV S gene was used to immunize mice orally.The content of SIgA in feces and the contents of IgG,IL-4,IL-2 and IFNγin serum of immunized mice were detected by ELISA,and the immune effect of Lactobacillus recombinant strain LP1522-PEDS on mice was evaluated.The results showed that after the first immunization,the contents of SIgA in feces and IgG,IL-4,IL-2 and IFN-γin serum of the commercial inactivated vaccine group and the engineered strain immunization group were significantly higher than those of the control group(P<0.05).The antibody levels of the inactivated vaccine group and the recombinant strain group reached the maximum after 42-56 days of immunization.The levels of antibodies and immune factors were as follows:commercial inactivated vaccine group>LP1522-PEDS group>empty vector group≈control group.Subsequently,the antibody levels in the inactivated vaccine group and LP1522-PEDS group gradually decreased.After 70 days of immunization,the antibody level of the commercial inactivated vaccine group decreased with small amplitude and the antibody level of the engineered strain immunization group decreased greatly.The results showed that the genetic engineering Lactobacillus plantarum LP1522-PEDS carrying PEDV S gene could induce PEDV immune response in mice via oral administration.

关 键 词：PEDV 乳酸菌 活载体疫苗 小鼠免疫 

分 类 号：S1[农业科学—农业基础科学]