基于分子模拟筛选优化玉米赤霉烯酮特异性多肽的荧光检测方法  

Optimization of Fluorescence Detection Method of Zearalenone Specific Polypeptide Based on Molecular Simulation

作　　者：陈雷 左文鹏 赵雷兴 黄信娥 李亦宸 牛富申 王宏利 赵晓丹 刘冰[1,2] CHEN Lei;ZUO Wenpeng;ZHAO Leixing;HUANG Xin'e;LI Yichen;NIU Fushen;WANG Hongli;ZHAO Xiaodan;LIU Bing(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]天津科技大学食品营养与安全国家重点实验室,天津300457

出　　处：《食品研究与开发》2024年第13期157-165,共9页Food Research and Development

基　　金：国家重点研发计划项目(2018YFC1604003)。

摘　　要：该研究通过分子模拟技术优化筛选玉米赤霉烯酮(zearalenone,ZEN)特异性多肽,并建立快速监测体系,预防ZEN中毒。分别用异硫氰酸荧光素和量子点标记模拟肽基于荧光共振能量转移的原理建立ZEN快速检测体系,基于荧光共振能量转移异硫氰酸检测方法,ZEN浓度0.1~40.0μg/L,线性关系(R^(2)=0.9907),检出限(S/N=3)为0.04μg/L。基于荧光共振能量转移量子点检测方法,ZEN浓度对数在0.08~65.00μg/L,线性关系(R^(2)=0.9929),检出限为0.03μg/L。对两种方法进行的特异性和稳定性评价显示出良好的结果。The molecular simulation technique was employed to optimize the detection method of the specific polypeptides of zearalenone(ZEN).A rapid detection system was established to prevent the poisoning caused by ZEN.The ZEN rapid detection system was established based on the principle of fluorescence resonance en⁃ergy transfer by using fluorescein isothiocyanate and quantum dot⁃labeled simulated peptides,respectively.The method established based on fluorescence resonance energy transfer with isothiocyanate labeling showed good linear relationship(R^(2)=0.9907)within the ZEN concentration of 0.1⁃40.0μg/L and limit of detection(S/N=3)being 0.04μg/L.The method established based on the fluorescence resonance energy transfer with quantum dot labeling showed good linear relationship(R^(2)=0.9929)within the ZEN concentration of 0.08⁃65.00μg/L and the limit of detection being 0.03μg/L.Both methods demonstrated high specificity and stability.

关 键 词：荧光共振能量转移 玉米赤霉烯酮 模拟肽 量子点 异硫氰酸 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]