C14-HSL limits the mycelial morphology of pathogen Trichosporon cells but enhances their aggregation:Mechanisms and implications  

作　　者：Xin Lu Haoran Sun Xiaomeng Li Chunrui Li Jinfeng Wang Dandan Zhou 

机构地区：[1]Jilin Engineering Lab for Water Pollution Control and Resources Recovery,School of the Environment,Northeast Normal University,Changchun 130117,China [2]State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210023,China

出　　处：《Chinese Chemical Letters》2024年第6期398-403,共6页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.52070036,U20A20322);the Fundamental Research Funds for the Central Universities(No.2412018ZD042)for their financial support。

摘　　要：The biosecurity hazards caused by pathogenic fungus have been widely concerned.Given the long-term coexistence of eukaryotic pathogens and quorum sensing bacteria in different habitats in environments,we hypothesized that they have social interactions via signal molecules.In this work,we firstly discovered the well-known bacterial signal molecules play an adverse role in the cell morphology and metabolism in a model pathogen Trichosporon asahii.N-Tetradecanoyl-L-homoserine lactone(C14-HSL)was discovered to increase pathogen hazards of T.asahii,which limited mycelium by 52%,but enhanced cell aggregation by 93%.Higher fluorescence intensity of tryptophan(59%)and aromatic protein(2-fold)contents after the treatment of C14-HSL,indicating that aromatic proteins helped aggregate Trichosporon and showed hydrophobicity.Transcriptome analysis revealed that C14-HSL upregulated the shikimate pathway(above1-fold)located in downstream of tricarboxylic acid cycle,which contributed to the synthesis of more aromatic proteins and the formation of larger flocs.The limited mycelial growth of T.asahii attributed to the up-regulated expressions of cell cycle process.The fungal transboundary response to bacterial C14-HSL was controlled by signal transduction pathways.This study provides new insights into the co-evolution of bacterial and pathogenic fungi in microecosystems.

关 键 词：Bacterial C14-HSL MYCELIUM Cell cycle AGGREGATION Aromatic proteins 

分 类 号：Q93[生物学—微生物学]