Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging  

作　　者：Boran Cheng Lei Cao Chen Li Fang-Yi Huo Qian-Fang Meng Ganglin Tong Xuan Wu Lin-Lin Bu Lang Rao Shubin Wang 

机构地区：[1]Department of Oncology,Shenzhen Key Laboratory of Gastrointestinal Cancer Translational Research,Cancer Institute,Peking University Shenzhen Hospital,Shenzhen-Peking University-Hong Kong University of Science and Technology Medical Center,Shenzhen 518036,China [2]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518132,China [3]The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education,School&Hospital of Stomatology,Wuhan University,Wuhan 430079,China

出　　处：《Chinese Chemical Letters》2024年第6期433-438,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82222035 and 81602489);the Guangdong Basic and Applied Basic Research Foundation(Nos.2021A1515111036 and 2022A1515110308);the Medical Scientific Research Foundation of Guangdong Province of China(No.A2023274);the Basic Research Program of Shenzhen Innovation Council(No.JCYJ20210324105609024)。

摘　　要：As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emission have rarely been reported.In this work,we prepared deep-red emissive fluorine-doped carbon quantum dots(F-CDs)by introducing a precursor simultaneously containing fluorine and amidogen.The synergistic effect of nitrogen doping and D-π-A pattern production contributed to the maximum emission of F-CDs at 636 nm with an absolute quantum yield of 36.00%±0.68%.Moreover,we designed an F-CDs-based fluorescence assay to determine the content of hypochlorite(ClO^(-)),with a limit of detection(LOD)as low as 15.4 nmol/L,indicating the high sensitivity of F-CDs to ClO^(-).In real samples,the F-CDs-based fluorescent sensor exhibited excellent sensitivity and selectivity in the detection of ClO^(-),with an error below 2%,suggesting their great potential in daily life.In cancer cell imaging,the F-CDs not only demonstrated high sensitivity to ClO^(-)but also exhibited excellent mitochondria targeting,as evidenced by the high Pearson's correlation coefficient(PCC)of 0.93 in colocalization analysis.The work presented here suggests the great potential of replacing commercial dyes with F-CDs for highly specific mitochondria labeling and cell imaging.

关 键 词：Carbon dots Near-infrared Cell imaging Mitochondria labeling Hypochlorite determination 

分 类 号：R73-3[医药卫生—肿瘤] O657.3[医药卫生—临床医学] TB383.1[理学—分析化学]