The Mn/Yb/Er triple-doped CeO_(2) nanozyme with enhanced oxidase-like activity for highly sensitive ratiometric detection of nitrite  

作　　者：Simin Wei Yaqing Yang Junjie Li Jialin Wang Jinlu Tang Ningning Wang Zhaohui Li 

机构地区：[1]College of Chemistry,Institute of Analytical Chemistry for Life Science,Henan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical Applications,Zhengzhou University,Zhengzhou 450001,China [2]School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Chinese Chemical Letters》2024年第6期491-495,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22004111 and 21974125);the Tackle Key Problems in Science and Technology Project of Henan Province,China(No.222102310386);China Postdoctoral Science Foundation(No.2020M682327);Henan Postdoctoral Foundation(No.202002009);Program for Innovative Research Team(in Science and Technology)in University of Henan Province(No.22TRTSTHN002);Excellent Youth Foundation of Henan Scientific Committee(No.232300421021)。

摘　　要：Long-term excessive intake of nitrite(NO_(2)^(-))poses a great threat to human health,needing a simple and fast method to detect NO_(2)-in food.Herein,via a simple and feasible strategy,Mn/Yb/Er triple-doped CeO_(2) nanozyme(Mn/Yb/Er/CeO_(2))was synthesized for highly sensitive ratiometric detection of nitrite.By doping Mn,Yb,Er into CeO_(2) lattice structure,Mn/Yb/Er/CeO_(2) nanozyme showed enhanced oxidase-like activity,obtaining a higher density of oxygen vacancy and a higher ratio of Ce^(3+)to Ce~(4+)than that of CeO_(2).The 3,3,5,5-tetramethylbenzidine(TMB)can be effectively oxidized by Mn/Yb/Er/CeO_(2) to produce the oxidized TMB(ox TMB),showing a significant absorption signal at 652 nm.Additionally,nitrite can react with ox TMB to produce yellow diazotized ox TMB,which is accompanied by an elevated absorption signal at 445 nm and a decreased absorption signal at 652 nm.Thus,based on the oxidase-mimetic activity of Mn/Yb/Er/CeO_(2) and the diazotization reaction between NO_(2)^(-)and ox TMB,a ratiometric colorimetric assay was established for NO_(2)^(-)detection in food.Furthermore,by integrating Mn/Yb/Er/CeO_(2) with a smartphone,a colorimetric smartphone-sensing platform was successfully fabricated for visualization and quantitative detection of NO_(2)^(-).Notably,this two-detection mode showed excellent sensitivity,selectivity,reliability and practicability in monitoring the NO_(2)^(-)in real samples,impling its great potential for food safety.

关 键 词：Mn/Yb/Er/CeO_(2) nanozyme Oxidase-like activity NITRITE Ratiometric detection Two-detection mode 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]