冬化油中VC棕榈酸酯含量检测  

Detection of VC Palmitate Content in Winterized Oil

作　　者：陆姝欢 徐柳柳 李慕梓 舒敏 李博玲 李翔宇 LU Shuhuan;XU Liuliu;LI Muzi;SHU Min;LI Boling;LI Xiangyu(CABIO Bioengineering(Wuhan)Co.,Ltd.,Wuhan 430073;Hubei Province Chemicals Biosynthetic Engineering Technology Research Center,Wuhan 430223)

机构地区：[1]嘉必优生物技术(武汉)股份有限公司,武汉430073 [2]湖北省营养化学品生物合成工程技术研究中心,武汉430223

出　　处：《食品工业》2024年第5期299-302,共4页The Food Industry

摘　　要：为定量分析冬化油中VC棕榈酸酯添加情况,建立一种利用高效液相色谱法测定冬化油中VC棕榈酸酯含量的方法。以含1 mg/mL柠檬酸的甲醇为样品提取剂,经12 000 r/min,-20℃冷冻离心得上清液,以甲醇-水-乙酸(95︰5︰0.1, V/V)为流动相,流速1 mL/min,经反相C18色谱柱等度洗脱,用紫外检测器检测,最佳吸收波长250 nm。结果显示,方法定性检出限为0.5 mg/kg,定量限为1.5 mg/kg,加标回收率在88.18%~101.54%之间, SRSD在1.24%~6.97%之间。该方法不需要特殊溶剂和复杂反应就可以快速、高效完成冬化油中VC棕榈酸酯含量分析。To analyze the addition of VC palmitate in winterized oil quantitatively, establish a high-performance liquid chromatography method for determining the content of VC palmitate added in winterized oil. Use methanol containing 1 mg/m L citric acid as the sample extraction agent, use methanol-water-acetic acid(95︰5︰0.1, V/V) as the mobile phase. Flow rate is 1 mL/min, After equal elution on a reversed phase C_(18) chromatographic column and detection with a UV detector, the optimal absorption wavelength is 250 nm. The results indicate that the qualitative detection limit of the method is 0.5 mg/kg,and the quantitative limit is 1.5 mg/kg. The recovery rate of spiking is between 88.18% and 101.54%, SRSD is between 1.24%and 6.97%. This method can quickly and efficiently determine the content of VC palmitate in winterized oil without special solvents and complex reactions.

关 键 词：冬化油 VC棕榈酸酯 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS227[理学—化学]