无症状胶质瘤的差异性基因表达及其富集分析  

Differential gene expression and enrichment analysis in asymptomatic gliomas

作　　者：冯亚文[1] 毛冲冲[1] 耿俊杰[1] 邱锴[1] 赵海彪 刘献志[1] Feng Yawen;Mao Chongchong;Geng Junjie;Qiu Kai;Zhao Haibiao;Liu Xianzhi(Department of Neurosurgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院神经外科,郑州450052

出　　处：《中华实验外科杂志》2024年第5期1055-1057,共3页Chinese Journal of Experimental Surgery

摘　　要：目的:分析有无症状世界卫生组织(WHO)2级脑胶质瘤的差异表达基因和相关富集通路,为胶质瘤的综合诊疗寻找新的靶点。方法:选取于2018年1月至2019年12月间在郑州大学第一附属医院神经外科接受手术治疗的27例WHO 2级脑胶质瘤患者[年龄(42.1±11.2)岁]进行本次研究。获取其临床随访资料和测序数据,将其分为无症状组(9例)和有症状组(18例)。通过R语言软件对比分析两组患者基因表达的差异。通过Metascape网站进行富集分析寻找相关富集通路。结果:在27例WHO 2级胶质瘤患者中,共筛选出127个差异表达基因,其中52上调基因,75个下调基因,并且在"急性炎性反应"、"血液循环"、"硫化合物结合"和"神经活性配体-受体相互作用"等通路中富集。结论:无症状WHO 2级胶质瘤具有特异性的基因表达差异并存在相关富集通路。Objective To find a new target for the comprehensive diagnosis and treatment of gliomas,we analyzed the differentially expressed genes and related enrichment pathways of asymptomatic world health organization(WHO)grade 2 gliomas.Methods In total,27 patients aged(42.1±11.2)years old with WHO grade 2 glioma who received surgical treatment in Department of Neurosurgery of the First Affiliated Hospital of Zhengzhou University from January 2018 to December 2019 were selected for this study.The clinical follow-up data and sequencing data were obtained and divided into asymptomatic group(9 cases)and symptomatic group(18 cases).The difference in gene expression between the two groups was analyzed by R language software.The Metascape website enrichment analysis was done to find the relevant enrichment pathways.Results In 27 WHO grade 2 glioma patients,127 differentially expressed genes were screened,including 52 up-regulated genes and 75 down-regulated genes,which were enriched in biological pathways such as acute inflammatory response,blood circulation,sulfur compound binding,and neuroactive ligand receptor interaction.Conclusion Asymptomatic WHO grade 2 gliomas exhibit specific gene expression differences and related enrichment pathways.

关 键 词：神经胶质瘤 低级别 差异表达基因 富集分析 

分 类 号：R739.41[医药卫生—肿瘤]