DXR调控番茄叶绿体发育、花色与果实着色机制初探  

Preliminary Study on the Mechanism of DXR Regulating Chloroplast Development Flower Color and Fruit Coloring in Tomato

作　　者：马星云 范冰丽 唐光彩 贾芝琪[1] 李营 薛东齐 张世文 MA Xingyun;FAN Bingli;TANG Guangcai;JIA Zhiqi;LI Ying;XUE Dongqi;ZHANG Shiwen(International Joint Laboratory of Horticulture,College of Horticulture,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学园艺学院,园艺国际联合实验室,郑州450046

出　　处：《园艺学报》2024年第6期1241-1255,共15页Acta Horticulturae Sinica

基　　金：国家自然科学基金青年基金项目(31902024);河南省科技攻关项目(212102110413,202102110402);河南农业大学博士启动基金项目(30500495)。

摘　　要：在植物的生长发育过程中,类胡萝卜素对叶、花、果的着色有着重要影响,是果实发育过程中的主要色素物质。1–脱氧–D–木酮糖–5–磷酸还原异构酶基因(DXR)参与了类胡萝卜素的合成,构建番茄DXR基因编辑株系和超量表达株系,发现基因编辑株系叶片呈现黄色甚至白色,叶片的叶绿素含量和最大PSⅡ的光能转换效率显著降低,细胞叶绿体发育受到影响,类囊体数量减少;花瓣边缘白化,红熟期果实颜色变为橙色甚至黄色,果实番茄红素、类黄酮等含量有不同程度降低。而超量表达株系与野生型对照相比无明显差异,但可溶性固形物含量明显增多。通过转录组测序发现,SlDXR与类黄酮生物合成、类胡萝卜素生物合成等途径相关。推测SlDXR可能通过调控萜类化合物的合成来影响番茄果实着色。In the growth and development of plants,carotenoids play a crucial role in leaf,flower,and fruit pigmentation,serving as the primary pigment compounds during fruit maturation.The 1-deoxyd-D-xylose-5-phosphate reduction isomerase gene(DXR)is involved in the synthesis of carotenoids.The SlDXR gene editing and overexpression lines of tomato were constructed.It was found that gene editing lines exhibited yellow or even white leaves,the chlorophyll content and maximum photoenergy conversion efficiency of PSⅡdecreased significantly,the chloroplast development was affected,and the number of thylakoids was reduced.Meanwhile,the petal edge of gene editing lines turned white and the fruit color changed to orange or even yellow in the red ripening stage.The content of lycopene,flavonoid and other pigments in these fruits decreased to different degrees.There was no significant difference between the overexpression linesand the wild type(the control),however,the soluble solids content was significantly increased.Transcriptome sequencing showed that SlDXR was related to flavonoid biosynthesis,carotenoid biosynthesis and other pathways.These findings suggested that SlDXR could affect the coloring of tomato by regulating the synthesis of terpenoids.

关 键 词：番茄 DXR 基因编辑 叶绿素 番茄红素 果实颜色 

分 类 号：S641.2[农业科学—蔬菜学]