DNA倍体定量分析、HPV E6/E7 mRNA检测及HPV筛查在宫颈病变诊断中的价值  

Value Analysis of DNA Ploidy Quantification,HPV E6/E7 mRNA Detection and HPV Screening in the Diagnosis of Cervical Lesions

作　　者：蒋洪波 刘研 肖金银 JIANG Hongbo;LIU Yan;XIAO Jinyin(Department of Gynecology,Changning District Maternal and Child Health Hospital,Shanghai 200051,China)

机构地区：[1]上海市长宁区妇幼保健院妇科,上海200051

出　　处：《河南医学高等专科学校学报》2024年第3期299-302,共4页Journal of Henan Medical College

摘　　要：目的比较分析宫颈病变筛查中人乳头瘤病毒(human papilloma virus,HPV)DNA基因分型检测、DNA倍体定量分析、HPV E6/E7 mRNA检测的价值。方法选取宫颈病变筛查者258例作为研究对象,均接受HPV DNA基因分型检测、HPV E6/E7 mRNA、DNA倍体定量分析及宫颈组织病理检查,对比DNA倍体定量分析、HPV E6/E7 mRNA检测、HPV DNA基因分型检测与宫颈组织病理检查结果,统计分析DNA倍体定量分析、HPV E6/E7 mRNA检测、HPV DNA基因分型检测的价值。以宫颈组织病理检查为金标准。结果DNA倍体定量分析的灵敏性为70.00%(42/60),特异性为77.27%(153/198),准确性为75.58%(195/258),阳性预测值为48.28%(42/87),阴性预测值为89.47%(153/171)。HPV E6/E7 mRNA检测的灵敏性为86.67%(52/60),特异性为59.60%(118/198),准确性为65.89%(170/258),阳性预测值为39.39%(52/132),阴性预测值为93.65%(118/126)。HPV DNA基因分型检测的灵敏性为95.00%(57/60),特异性为39.39%(78/198),准确性为52.33%(135/258),阳性预测值为32.20%(57/177),阴性预测值为96.30%(78/81)。HPV DNA基因分型检测的灵敏性、阴性预测值均高于DNA倍体定量分析、HPV E6/E7 mRNA检测,特异性、准确性、阳性预测值均低于DNA倍体定量分析、HPV E6/E7 mRNA检测(P<0.05),HPV E6/E7 mRNA检测的灵敏性、阴性预测值均高于DNA倍体定量分析,特异性、准确性、阳性预测值均低于DNA倍体定量分析(P<0.05)。结论宫颈病变筛查中HPV DNA基因分型检测的价值高于DNA倍体定量分析及HPV E6/E7 mRNA检测,值得应用。Objective To comparatively analyze the value of HPV DNA genotyping,DNA ploidy quantification and HPV E6/E7 mRNA detection in cervical lesion screening.Methods A total of 258 cases of cervical lesion screeners were selected for the study and were examined for HPV DNA genotyping,HPV E6/E7 mRNA,DNA ploidy quantification and cervical histopathology.The results of the above examinations were analyzed for their application value through comparative statistics,and cervical histopathology was considered the gold standard.Results The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of DNA ploidy quantification were 70.00%(42/60),77.27%(153/198),75.58%(195/258),48.28%(42/87)and 89.47%(153/171),respectively.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of HPV E6/E7 mRNA detection were 86.67%(52/60),59.60%(118/198),65.89%(170/258),39.39%(52/132)and 93.65%(118/126),respectively.The sensitivity and negative predictive value of HPV DNA genotyping test were higher than those of quantitative DNA ploidy analysis and HPV E6/E7 mRNA test,but the specificity,accuracy and positive predictive value were lower than those of quantitative DNA ploidy analysis and HPV E6/E7 mRNA test(P<0.05).HPV E6/E7 mRNA testing had higher sensitivity and negative predictive value than DNA ploidy quantification,but had lower specificity,accuracy and positive predictive value(P<0.05).Conclusion The value of HPV DNA genotyping in cervical lesion screening is higher than that of DNA ploidy quantification and HPV E6E7 mRNA detection,and it is worthy of clinical promotion and application.

关 键 词：宫颈病变 筛查 HPV DNA基因分型检测 HPV E6/E7 mRNA检测 DNA倍体定量分析 

分 类 号：R737.33[医药卫生—肿瘤]