加味增液汤HPLC指纹图谱及3种主要成分的含量测定  

HPLC fingerprint and three main components determination of Modified Zengye Decoction

作　　者：刘诗琪 吴闯[1,2] 朱智敏 徐烽 常耀文 张海岩 苟小军[1,3] LIU Shiqi;WU Chuang;ZHU Zhimin;XU Feng;CHANG Yaowen;ZHANG Haiyan;GOU Xiaojun(Department of Proctology,Baoshan Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201999,China;Department of Proctology,Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 201999,China;Central Laboratory,Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 201999,China)

机构地区：[1]上海中医药大学附属宝山医院肛肠科,上海201999 [2]上海市宝山区中西医结合医院肛肠科,上海201999 [3]上海市宝山区中西医结合医院中心实验室,上海201999

出　　处：《中国药师》2024年第6期928-936,共9页China Pharmacist

基　　金：国家自然科学基金青年科学基金项目(81704078);“十四五”中医特色专科和中医急诊能力提升(第一批)项目(ZYTSKI-8);宝山区中西医结合医院优秀青年医学人才培养项目(2022BY002);上海市宝山区科学技术委员会医学卫生项目(2023-E-16)。

摘　　要：目的建立加味增液汤HPLC指纹图谱并对3种主要成分进行含量测定。方法色谱柱为岛津WondaSil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.3%磷酸水溶液,梯度洗脱;流速为1.0 mL/min;检测波长为265、203、310、290 nm;柱温为25°C;进样量为20μL。建立10批加味增液汤HPLC指纹图谱,采用中药色谱指纹图谱相似度评价系统软件(2012A版)进行相似度分析,对共有峰进行鉴定和药材归属,并测定3种主要成分的含量。结果10批加味增液汤的指纹图谱有17个共有峰,其相似度介于0.872~0.989之间。指认出9、14、17号峰分别为阿魏酸、橙皮苷、哈巴俄苷。阿魏酸、橙皮苷、哈巴俄苷的含量分别为0.0673~0.1748、0.4988~1.5227、0.2709~0.8024 mg/g,转移率分别为30.74%~55.63%、11.77%~35.94%、23.15%~68.56%。结论该研究所建立的HPLC指纹图谱结合主要成分定量分析方法可用于加味增液汤汤剂的质量分析与控制,分析方法简单,结果可靠。Objective To establish a method for HPLC fingerprint analysis and determine three main components of Modified Zengye Decoction.Methods The chromatographic column was Shimadzu WondaSil C18 column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.3%aqueous phosphoric acid with a gradient elution procedure,the volume flow rate was 1.0 mL/min,the detection wavelengths were 265,203,310 and 290 nm,the column temperature was 25°C,and the injection volume was 20μL.The HPLC fingerprints of the 10 batches of Modified Zengye Decoction were established,and the similarity analysis was performed by using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine(version 2012A).The common peaks were identified and assigned,and the contents of the three main components were quantitatively determined.Results There were 17 common peaks in the fingerprints of 10 batches of Modified Zengye Decoction with similarities ranging from 0.872-0.989.The fingerprints recognized peak 9,14 and 17 as ferulic acid,aurantiamarin and harpagoside,respectively.The contents of ferulic acid,aurantiamarin and harpagoside were 0.0673-0.1748,0.4988-1.5227,0.2709-0.8024 mg/g,and the transfer rate were 30.74%-55.63%,11.77%-35.94%,23.15%-68.56%,respectively.Conclusion The established HPLC fingerprint analysis method combined with main components quantitative analysis method can be used for the quality analysis and control of Modified Zengye Decoction with simple analysis method and reliable results.

关 键 词：加味增液汤 阿魏酸 橙皮苷 哈巴俄苷 高效液相色谱法 指纹图谱 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]