NK细胞体外扩增的影响因素及临床应用  

The influencing factors and clinical applications of NK cell expansion in vitro

作　　者：刘金影 庞晶晶 高旭 寇佳媛 张艳芬[1] LIU Jinying;PANG Jinging;GAO Xu;KOU Jiayuan;ZHANG Yanfen(Department of Clinical Laboratory,the Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China;Department of Biochemistry and Molecular Biology,Basic Medical College,Harbin Medical University,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属第二医院检验科,哈尔滨150081 [2]哈尔滨医科大学基础医学院生物化学与分子生物学教研室,哈尔滨150081

出　　处：《生命的化学》2024年第5期900-909,共10页Chemistry of Life

基　　金：哈尔滨医科大学附属第二医院中青年创新科学研究基金项目(KYCX2019-14);国家自然科学基金项目(82300511);2022年度黑龙江省留学回国人员择优资助项目。

摘　　要：随着免疫学及分子生物学技术的发展,以细胞为主体的(如T细胞、NK细胞等)生物疗法在肿瘤过继性细胞免疫治疗中显示出巨大的潜能。与T细胞疗法相比,NK细胞无需激活即可完成杀伤,具有快速释放炎性细胞因子和杀死靶细胞的能力,因此近年来受到格外关注。但人体内NK细胞的数量、纯度和活性无法满足科研及临床应用的需要,阻碍了以NK细胞为基础的多种应用的发展,故有必要在体外对NK细胞进行有效扩增。本文从NK细胞体外扩增的影响因素及其临床应用两方面进行综述。T With the development of immunology and molecular biology technology,cell based therapies(such as T cells,NK cells,etc.)have shown great potential in adoptive cell immunotherapy for tumors.Compared with T cell therapy,NK cells can complete killing without activation and have the ability to quickly release inflammatory cytokines and kill target cells,thus receiving special attention in recent years.However,the number,purity,and activity of NK cells in the human body cannot meet the needs of scientific research and clinical applications,which hinders the development of various applications based on NK cells.Therefore,it is necessary to effectively expand NK cells in vitro.This paper reviews the influencing factors and clinical applications of NK cell expansion in vitro.

关 键 词：NK细胞 体外扩增 临床应用 

分 类 号：R730.51[医药卫生—肿瘤]