基于DNAzyme辅助信号放大的荧光检测APE1  

Fluorescence Assay for APE1 Based on DNAzyme Assisted Cascade Amplification

作　　者：葛永浩 宋盼 张晓茹[1] 宋维玲 GE Yonghao;SONG Pan;ZHANG Xiaoru;SONG Weiling(College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China)

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2024年第3期31-36,共6页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(21705086)。

摘　　要：利用脱氧核酶(DNAzyme)辅助放大荧光信号,发展了一种简便快速无嘌呤/无嘧啶内切酶(APE1)的高灵敏度检测策略。验证了实验可行性,对反应时间、反应温度进行了优化,在最优条件下对目标物APE1进行了检测,检测限为3.0×10^(-4)U·mL^(-1),并证明了本实验具有良好的选择性。In this study,the DNAzyme assisted signal amplification strategy was developed for simple,rapid and highly sensitive detection of APE1.The experiment conditions including reaction time and temperature were optimized.This method exhibits feasibility,excellent specificity and high sensitivity with a detection limit of 3.0×10^(-4) U·mL^(-1).

关 键 词：DNAZYME 荧光 APE1 信号放大 

分 类 号：O657.3[理学—分析化学]