马铃薯晚疫病抗性基因R8分子标记检测与田间抗性评价  

Molecular Marker Screening of Potato Late Blight Resistant Gene R8 and Evaluation of Field Resistance

作　　者：娄树宝[1,2] 杨梦平 邢金月[1] 翟玲侠 王辉 刘春生[1] 王立春[1] 宋继玲[1] Lou Shubao;Yang Mengping;Xing Jinyue;Zhai LingxiaWang Hui;Liu Chunsheng;Wang Lichun;Song Jiling(Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Test-Tube Plantlet Bank of National Potato Germplasm Resources(Keshan),Qiqihar 161005,Heilongjiang,China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]黑龙江省农业科学院克山分院/国家马铃薯种质资源试管苗库(克山),黑龙江齐齐哈尔161005 [2]青海大学农林科学院,青海西宁810016

出　　处：《作物杂志》2024年第3期247-251,共5页Crops

基　　金：齐齐哈尔市科技计划创新激励项目(CNYGG-2022025);科技部、财政部、国家科技资源共享服务平台项目“国家作物种质资源库马铃薯分库运行服务”(NCGRC-2022-44);农业农村部物种保护项目“马铃薯种质资源安全保存及普查收集资源鉴定评价与繁殖编目入库”(19221860);农业农村部“马铃薯种质资源精准鉴定”(19221974)。

摘　　要：马铃薯是重要的粮食作物,在全球粮食安全中发挥着重要作用。由致病疫霉(Phytophthora infestans)引起的晚疫病是马铃薯生产上最具危害性的病害之一,种植广谱和持久抗病马铃薯品种是防治晚疫病最经济、有效和绿色的方法。本研究利用特异性分子标记对102份资源进行R8基因检测,结合田间晚疫病抗性鉴定,筛选出84份表现抗病并且含有R8基因的马铃薯材料,为选育抗病品种提供丰富的资源材料;根据分子标记与田间抗性鉴定结果,分子标记抗性符合度为84.8%,因此R8分子标记可以用于分子标记辅助选择。Potato is an important food crop that plays an important role in global food security.Late blight caused by Phytophthora infestans is one of the most devastating diseases in potato production,and planting broad-spectrum and durable resistant varieties is the most economical,effective and green way to control late blight.In this study,102 resources were conduced for R8 gene using specific molecular markers,and 84 potato materials with disease resistance and containing R8 gene were selected in combination with field late blight resistance identification,providing abundant resource materials for selection and breeding of disease resistant varieties.According to the results of molecular marker and field resistance identification,the molecular marker resistance compliance was 84.8%,so the R8 molecular marker could be used for marker-assisted selection.

关 键 词：马铃薯 晚疫病 分子标记 田间抗性 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]