瑞香素调控DJ-1表达对子宫内膜癌Ishikawa细胞凋亡的影响  

Effect of Ruscogenin regulation on DJ-1 expression on apoptosis in endometrial cancer Ishikawa cells

作　　者：龙生根[1] 章志琴 朱其舟[1] 肖仲清[1] 舒宽勇 LONG Sheng-gen;ZHANG Zhi-qin;ZHU Qi-zhou;XIAO Zhong-qing;SHU Kuan-yong(Department of Oncology,Jiangxi Maternal and Child Health Hospital,Nanchang 330000,Jiangxi,China;不详)

机构地区：[1]江西省妇幼保健院肿瘤科,江西南昌330000 [2]江西省妇幼保健院辅助生殖中心,江西南昌330000

出　　处：《广东医学》2024年第5期529-533,共5页Guangdong Medical Journal

基　　金：国家自然科学基金委员会地区科学基金项目(82360318);江西省中医药管理局科技计划项目(2022B913)。

摘　　要：目的探讨中药活性成分瑞香素调控DJ-1表达对子宫内膜癌Ishikawa细胞凋亡的影响。方法以子宫内膜癌Ishikawa细胞为研究对象,CCK-8法分析瑞香素对Ishikawa细胞增殖的影响,qRT-PCR检测瑞香素对Ishikawa细胞DJ-1 mRNA表达,流式细胞术检测瑞香素对Ishikawa细胞凋亡的影响。结果不同浓度瑞香素处理Ishikawa细胞后,细胞增殖抑制率较对照组明显增加,且呈现时间-浓度依赖性,瑞香素处理Ishikawa细胞48 h,抑制率IC50值为78μmol/L;与对照组相比,78μmol/L瑞香素处理Ishikawa细胞48 h,其DJ-1mRNA表达量明显降低(P<0.05),同时早期凋亡率(P<0.01)和晚期凋亡率(P<0.05)均明显升高。结论瑞香素可通过降调DJ-1的表达促进子宫内膜癌Ishikawa细胞凋亡,并呈剂量依赖关系。Objective To investigate the effect of the active component of traditional Chinese medicine,ruscogenin,on the expression of DJ-1 and apoptosis in endometrial cancer Ishikawa cells.Methods Endometrial cancer Ishikawa cells were used as the research objects.The CCK-8 method was used to analyze the effect of ruscogenin on the proliferation of Ishikawa cells.qRT-PCR was used to detect the expression of DJ-1 mRNA in Ishikawa cells treated with ruscogenin,and flow cytometry was used to detect the effect of ruscogenin on apoptosis in Ishikawa cells.Results After treatment with different concentrations of ruscogenin,the inhibition rate of cell proliferation in Ishikawa cells was significantly increased compared to the control group,showing a time-concentration-dependent manner.After treatment with ruscogenin for 48 hours,the IC50 value of the inhibition rate was 78μmol/L.Compared with the control group,the expression level of DJ-1 mRNA in Ishikawa cells treated with 78μmol/L ruscogenin for 48 hours was significantly decreased(P<0.05),while the early apoptosis rate(P<0.01)and late apoptosis rate(P<0.05)were significantly increased.Conclusion Ruscogenin can promote apoptosis in endometrial cancer Ishikawa cells by downregulating the expression of DJ-1,and this effect is dose-dependent.

关 键 词：瑞香素 子宫内膜癌 ISHIKAWA细胞 DJ-1 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤] R28[医药卫生—临床医学]