基于氧化应激介导NF-κB通路研究ALDH2基因表达对氯胺酮相关性膀胱炎的影响  

Effect of ALDH2 gene expression on ketamine-induced cystitis based on oxidative stress-mediated NF-κB pathway

作　　者：章民昊 奚晓剑 顾鹏 何晓亮 ZHANG Minhao;XI Xiaojian;GU Peng;HE Xiaoliang(Department of Urology,Xishan People’s Hospital of Wuxi City,Wuxi 214000,China)

机构地区：[1]江苏省无锡市锡山人民医院泌尿外科,无锡214000

出　　处：《广西医科大学学报》2024年第5期728-733,共6页Journal of Guangxi Medical University

基　　金：江苏省无锡市卫生健康委科研青年项目(No.Q202030)。

摘　　要：目的:研究乙醛脱氢酶(ALDH2)基因表达对氧化应激介导的NF-κB通路在氯胺酮相关性膀胱炎(KIC)中的影响。方法:将SV-HUC-1细胞分为不同浓度氯胺酮组(0 mmol/L、0.5 mmol/L、1 mmol/L、2 mmol/L),诱导48 h后检测ALDH2蛋白表达量;然后将SV-HUC-1细胞构建分为对照组(si-NC组、OE-NC组)、ALDH2沉默组(si-ALDH2组)、ALDH2过表达组(OE-ALDH2组);分别用完全培养基和氯胺酮(1 mmol/L)诱导刺激细胞48 h,检测每组细胞内炎症因子和炎症蛋白水平。结果:蛋白质免疫印迹法(western blotting)显示经氯胺酮(0.5 mmol/L、1 mmol/L)处理后的ALDH2蛋白相对表达量显著高于0 mmol/L组(P<0.05);进一步在氯胺酮(1 mmol/L)诱导后的组中比较发现,si-ALDH2组炎症细胞因子白细胞介素-1β(IL-1β)及白细胞介素-6(IL-6)的表达较si-NC组显著增加(P<0.05);OE-ALDH2组IL-1β及IL-6的表达较OE-NC组显著减少(P<0.05);si-ALDH2组中炎症蛋白核因子-κB(NF-κB)、环氧合酶-2(COX-2)、诱导型一氧化氮合酶(iNOS)的表达量均较si-NC组升高(P<0.05);OE-ALDH2组中炎症蛋白NF-κB、COX-2、iNOS的表达量均较OE-NC组降低(P<0.05)。而在对照组(0 mmol/L)中,siALDH2组与si-NC组,OE-ALDH2组与OE-NC组比较,炎症因子和炎症蛋白水平比较均无统计学差异(P>0.05)。结论:ALDH2基因可在KIC的发生中发挥一定的抗炎保护作用。Objective:To investigate the effect of acetaldehyde dehydrogenase 2(ALDH2)gene expression on oxidative stress-mediated NF-κB pathway in ketamine-induced cystitis(KIC).Methods:SV-HUC-1 cells were divided into different concentrations of ketamine groups(0 mmol/L,0.5 mmol/L,1 mmol/L,2 mmol/L),and the expression of ALDH2 protein was detected after induction for 48 hours.The SV-HUC-1 cell constructs were divided into control group(si-NC group,OE-NC group),ALDH2 silencing group(Si-ALDH2 group),and ALDH2 overexpression group(OE-ALDH2 group).The cells were induced with complete medium and ketamine(1 mmol/L)for 48 hours respectively,and the levels of inflammatory factors and inflammatory proteins were detected in each group.Results:Western blotting showed that the relative expression of ALDH2 protein after treatment with ketamine(0.5 mmol/L,1 mmol/L)was significantly higher than that in the 0 mmol/L group(P<0.05).In the further comparison of ketamine-induced group(1 mmol/L),it was found that the expression of inflammatory cytokines interleukin-1β(IL-1β)and interleukin-6(IL-6)in the si-ALDH2 group was significantly higher than that in the si-ALDH2 group(P<0.05);the expression of IL-1βand IL-6 in the OE-ALDH2 group was significantly lower than that in the OE-NC group(P<0.05).The expression levels of inflammatory protein nuclear factor-κB(NF-κB),cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)in the si-ALDH2 group were higher than those in the si-NC group(P<0.05).The expression levels of inflammatory proteins NF-κB,COX-2 and iNOS in the OE-ALDH2 group were lower than those in the OE-NC group(P<0.05).In the control group(0 mmol/L),there were no significant differences in the levels of inflammatory factors and inflammatory proteins between si-ALDH2 group and si-NC group,as well as OE-ALDH2 group and OE-NC group(P>0.05).Conclusion:ALDH2 gene may play a certain anti-inflammatory protective role in the occurrence of KIC.

关 键 词：氯胺酮相关性膀胱炎 乙醛脱氢酶 氧化应激 

分 类 号：R694.3[医药卫生—泌尿科学]