蛋白-O-岩藻糖基转移酶1在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of protein O-fucosyltransferase 1 in non-small cell lung cancer

作　　者：王晓慧 李明泰 高绿叶 郝艳龙 冯雪飞 翟元芳 郭艳琳[1] WANG Xiao-hui;LI Ming-tai;GAO Lv-ye;HAO Yan-long;FENG Xue-fei;ZHAI Yuan-fang;GUO Yan-lin(Department of Pathology,Shanxi Medical University,Taiyuan,Shanxi 030001,China;不详)

机构地区：[1]山西医科大学基础医学院病理教研室,山西太原030001 [2]山西医科大学基础医学院解剖教研室

出　　处：《现代预防医学》2024年第12期2248-2252,2304,共6页Modern Preventive Medicine

基　　金：山西省基础研究计划(自由探索类)(20210302124185);山西省回国留学人员科研资助项目(2021-082)。

摘　　要：目的 探讨非小细胞肺癌中蛋白-O-岩藻糖基转移酶1的表达及其临床意义,为非小细胞肺癌的诊断和治疗提供参考依据。方法 通过TCGA、UALCAN数据库和免疫组化技术探讨POFUT1 mRNA和POFUT1蛋白在非小细胞肺癌的表达情况。提取TCGA数据库的临床数据,采用Kaplan-Meier法进行生存分析,采用COX比例风险回归模型分析POFUT1 mRNA表达水平对非小细胞肺癌患者预后的影响。结果 POFUT1 mRNA和POFUT1蛋白在肺腺癌和肺鳞癌组织中均高表达(均P<0.05)。肺腺癌中POFUT1 mRNA的表达水平与临床分期相关(P<0.001),肺鳞癌中POFUT1 mRNA的表达与肿瘤直径相关(P<0.001)。COX回归分析结果显示,POFUT1 mRNA高表达(P=0.012)、肿瘤直径、临床分期、淋巴结转移与肺腺癌患者预后相关(均P<0.001),临床Ⅲ-Ⅳ期是肺腺癌患者独立危险因素。肿瘤直径(P=0.001)、临床Ⅲ-Ⅳ期(P=0.006)和POFUT1 mRNA高表达(P=0.049)与肺鳞癌患者预后相关,肿瘤直径大于5cm和POFUT1高表达是肺鳞癌患者独立危险因素。生存分析结果显示POFUT1高表达的非小细胞肺癌患者生存率显著低于低表达患者。结论 POFUT1在非小细胞肺癌组织中高表达,且其高表达与患者不良预后相关,可作为非小细胞肺癌预后评估的生物标志物。Objective To investigate the expression and clinical significance of protein-O-fucosyltransferase 1 in non-small cell lung cancer(NSCLC),so as to provide a reference for the diagnosis and treatment of NSCLC.Methods The expression of POFUT1in NSCLC was determined by TCGA,UALCAN database and immunohistochemistry.Based on the clinical data form TCGA database were extracted for survival analysis by Kaplan-Meier method,and analysis of the effect of POFUT1 mRNA expression level on prognosis of patients with NSCLC.Results POFUT1 was highly expression in lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC)tissues(all P<0.05).The expression level of POFUT1 mRNA in LUAD was correlated with clinical stage(P<0.001)and that in LUSC was correlated with tumor size(P<0.001).COX regression analysis showed that high mRNA expression of POFUT1(P=0.012),tumor size,clinical stage and lymph node metastasis were associated with the prognosis of LUAD patients(all P<0.001),and clinical stage III-IV was an independent risk factor for LUAD patients.Tumor size(P=0.001),clinical stage III-IV(P=0.006)and high expression of POFUT1 mRNA(P=0.049)were associated with the prognosis of LUSC patients,and tumor size greater than 5cm and high expression of POFUT1 were independent risk factors for LUSC patients.The survival analysis result showed that the survival rate of patients with high expression of POFUT1 was significantly lower than that of patients with low expression.Conclusion POFUT1 is highly expressed in NSCLC tissues,and high POFUT1 expression is correlated with poor prognosis in patients.POFUT1 can serve as a biomarker for prognostic evaluation of NSCLC.

关 键 词：非小细胞肺癌 POFUT1 生物信息学 免疫组织化学 预后 

分 类 号：R734.2[医药卫生—肿瘤]