虾青素对2型糖尿病小鼠术后认知功能障碍的影响  

Effect of astaxanthin on postoperative cognitive dysfunction in type 2 diabetes mellitus mice

作　　者：贾一帆[1] 杨帆[2] 陈璟莉 叶济世 万里鹏[2] JIA Yifan;YANG Fan;CHEN Jingli;YE Jishi;WAN Lipeng(Department of Pain Medicine,Renmin Hospital of Wuhan University,Wuhan,Hubei 430060,China;Department of Pain Medicine,Huanggang Central Hospital,Huanggang,Hubei 438000,China;Department of Anesthesiology,Wuhan Central Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430000,China)

机构地区：[1]武汉大学人民医院疼痛科,湖北武汉430060 [2]黄冈市中心医院疼痛科,湖北黄冈438000 [3]华中科技大学同济医学院附属武汉市中心医院麻醉科,湖北武汉430000

出　　处：《中华实用诊断与治疗杂志》2024年第6期541-545,共5页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金青年项目(82101253);湖北省自然科学基金面上项目(2022CFB108);武汉市科技局知识创新专项基础研究项目(2022020801010535);吴阶平医学基金会临床科研专项资助基金(320.6750.2024-11-01)。

摘　　要：目的 探讨虾青素对2型糖尿病小鼠术后认知功能障碍(POCD)的改善作用及可能机制。方法 雄性C57BL/6J小鼠48只,采用高脂饲料喂养+腹腔注射烟酰胺及链脲佐菌素法制备2型糖尿病模型。36只造模成功小鼠随机分为对照组、模型组、虾青素组各12只,模型组、虾青素组采用七氟醚麻醉下行剖腹探查术制备POCD模型,对照组正常饲养不行剖腹探查术。虾青素组术后腹腔注射虾青素30 mg/(kg·d),对照组、模型组腹腔注射等量生理盐水,共3 d;比较3组小鼠术前体质量、血糖水平及术后游泳速度。造模后采用Morris水迷宫实验评估小鼠术后认知功能(逃避潜伏期、穿越平台次数、目标象限停留时间百分比),采用免疫荧光染色法检测小鼠海马组织离子钙结合衔接分子1(Iba1)相对荧光强度及A1型星形胶质细胞百分比,采用ELISA法检测小鼠海马组织肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1α、补体1q(C1q)水平,采用Western blot法检测小鼠海马组织突触后致密蛋白95(PSD95)、突触素(SYP)蛋白相对表达量。结果 3组术前体质量、血糖水平及术后游泳速度比较差异均无统计学意义(P>0.05)。模型组逃避潜伏期[(34.58±10.54)s]长于对照组[(15.56±5.73)s]、虾青素组[(24.63±7.79)s](P<0.05),穿越平台次数[(2.92±1.08)次]、目标象限停留时间百分比[(29.04±10.30)%]均少于对照组[(6.25±2.09)次、(54.40±10.16)%]、虾青素组[(5.33±1.50)次、(41.04±9.20)%](P<0.05);虾青素组逃避潜伏期长于对照组(P<0.05),目标象限停留时间百分比少于对照组(P<0.05),穿越平台次数与对照组比较差异无统计学意义(P>0.05)。模型组海马组织Iba1相对荧光强度[(7 951.09±1 818.78)AU]、A1型星形胶质细胞百分比[(50.93±8.84)%]及TNF-α[(59.27±21.44)pg/mg prot]、IL-1α[(22.94±6.81)pg/mg prot]、C1q[(9.35±3.62)pg/mg prot]水平均高于对照组[(3 038.24±301.92)AU、(18.72±3.06)%、(20.26±6.86)pg/mg prot、(7.96±1.7Objective To investigate the ameliorative effects and possible mechanisms of astaxanthin on postoperative cognitive dysfunction(POCD)in type 2 diabetes mellitus mice.Methods Forty-eight male C57BL/6J mice received high-fat diet and were intraperitoneally injected with nicotinamide and streptozotocin to prepare type 2 diabetes mellitus models.Thirty-six mice models were randomly divided into control,model and astaxanthin groups,with 12 mice in each group.The POCD models were constructed by laparotomy under sevoflurane anesthesia in model and astaxanthin groups,and control group only received normal diet without laparotomy.Astaxanthin group was injected intraperitoneally with30 mg/(kg·d)of astaxanthin,while control and model groups were injected with an equal volume of normal saline,totally for 3 d.The body mass and plasma glucose before laparotomy,and swimming speed after laparotomy were compared among three groups.After modeling,Morris water maze test was done to assess the cognitive function(escape latency,number of platform crossings,and percentage of time spent in the target quadrant),and immunofluorescence was used to detect the relative fluorescence intensity of ionized calcium-binding adaptor molecule 1(Iba1)and the percentage of Al astrocytes in the hippocampus,the levels of tumor necrosis factor(TNF)-α,interleukin(IL)-1α,and complement1q(C1q)in the hippocampus were detected by ELISA,and the relative expressions of postsynaptic density protein(PSD)95 and synaptophysin(SYP)protein in the hippocampus were detected by Western blot.Results There were no significant differences in the preoperative body mass,preoperative plasma glucose and postoperative swimming speed among three groups(P>0.05).The escape latency was longer in model group[(34.58±10.54)s]than that in astaxanthin group[(24.63±7.79)s]and control group[(15.56±5.73)s](P<0.05),and longer in astaxanthin group than that in control group(P<0.05).The number of platform crossings and the percentage of time spent in the target quadrant were less in mo

关 键 词：术后认知功能障碍 2型糖尿病 虾青素 A1型星形胶质细胞 突触 小鼠 

分 类 号：R587.2[医药卫生—内分泌] R749.1[医药卫生—内科学]