Double-stranded DNA-nucleic acid dye complex-sensitized biocatalytic photosynthesis:Base pairing-switched regeneration of nicotinamide cofactor  

作　　者：Zhen Dong Yanying Wang Dan Li Peng Wu 

机构地区：[1]College of Chemistry,Sichuan University,Chengdu 610064,Sichuan,China [2]Analytical&Testing Centre,State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,Sichuan,China [3]School of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,Henan,China

出　　处：《Journal of Energy Chemistry》2024年第7期618-624,共7页能源化学（英文版）

基　　金：the financial support from the National Natural Science Foundation of China (22274102 and 22325403);the Fundamental Research Funds from Sichuan University (2022SCUNL104);the Open Research Fund of School of Chemistry and Chemical Engineering,Henan Normal University(2022A02)

摘　　要：Photo-biocatalysis,the combination of photosensitization and biocatalysis,is an emerging solution for sunlight-based renewable energy.It is thus important to develop light antennas with both good light har-vesting and efficient electron transfer.Herein,the intriguing electrical conductivity of dsDNA and its host effect(for nucleic acid dyes to harvest light)were explored simultaneously to develop a dsDNA-based light antenna for photo-biocatalysis.With SYBR Green I(SG)as the example of the nucleic acid dye,the proposed SG-dsDNA system was found to be capable for visible-light-driven reduced nicotinamide adenine dinucleotide(NADH)regeneration,and the turnover frequency of which(1.35 min^(-1))exceeded most of the existing photocatalytic systems.Since SG can only be hosted by dsDNA,meanwhile dsDNA can be formed through hybridization between single strand DNA and its complementary strand,the pro-posed system adds an extra control of the photocatalytic activity(DNA base pairing-based switch).When integrating the SG-dsDNA system with NADH-dependent horse liver alcohol dehydrogenase(HLADH),successful synthesis of 2-phenylpropanol(a crucial intermediates of profens manufacturing)was achieved.

关 键 词：PHOTOSYNTHESIS DSDNA SYBRGreenI TICT 2-phenylpropanol 

分 类 号：Q503[生物学—生物化学] O643.3[理学—物理化学]