高效液相色谱法测定天然彩色茧中叶黄素  

Determination of lutein in natural color cocoons by high performance liquid chromatography

作　　者：严颖鹏 吴娟 陈玲玲 YAN Yingpeng;WU Juan;CHEN Lingling(Zhejiang Academy of Science&Technology for Inspection&Quarantine,Hangzhou Zhejiang,310012,China)

机构地区：[1]浙江省检验检疫科学技术研究院,浙江杭州310012

出　　处：《质量安全与检验检测》2024年第3期10-12,56,共4页QUALITY SAFETY INSPECTION AND TESTING

摘　　要：本研究采用超声波萃取,高效液相色谱法测定天然彩色茧中叶黄素,色谱柱为ZORBAZ Extend C_(18)(4.6×150 mm,5μm),流动相为甲醇∶水(95∶5),流速为1 mL/min,检测波长为450 nm。结果显示,叶黄素浓度在0.5μg/mL~10μg/mL范围内呈线性,相关系数R^(2)=0.999,方法回收率为96%~100%,相对标准偏差为0.33%,表明该方法可用于天然彩色茧中叶黄素的测定。The lutein in natural color cocoons was extracted by ultrasonic and detected by high performance liquid chromatography,the chromatography was performed on ZORBAZ Extend C_(18)(4.6×150 mm,5μm)with mobile phase consisting of methanol∶water(95∶5),the flow rate was 1 mL/min,the detection wavelength was 450 nm.The results showed that the lutein concentration exhibits linearity within the range of 0.5μg/mL to 10μg/mL,with a correlation coefficient R^(2) of 0.999,the recovery was 96%-100%,and the relative standard deviation was 0.33%,it is thus clear that the method could be used for the determination of lutein in natural color cocoons.

关 键 词：叶黄素 天然彩色茧 高效液相色谱 

分 类 号：O657.72[理学—分析化学]