12C^(6+)辐射对菊苣根黄酮类物质及抗氧化活性的影响  

Effect of 12C^(6+)Radiation on Flavonoids and Antioxidant Activity in Chicory Roots

作　　者：唐君蔚 曹天光[1] 秦垒 王慧[1] 范云鹏 刘丽丽[1] 耿金鹏[1] TANG Junwei;CAO Tianguang;QIN Lei;WANG Hui;FAN Yunpeng;LIU Lili;GENG Jinpeng(Institution of Biophysics,Hebei University of Technology,Tianjin 300401)

机构地区：[1]河北工业大学生物物理研究所,天津300401

出　　处：《核农学报》2024年第8期1434-1442,共9页Journal of Nuclear Agricultural Sciences

基　　金：河北省科技计划结转项目(19227527D);河北省科技计划项目(21326342D);河北省重点研发计划项目(21326312D)。

摘　　要：为探究12C^(6+)辐射对菊苣中黄酮类物质及抗氧化活性的影响,以菊苣根为试验材料,测定不同12C^(6+)辐射剂量下总黄酮含量及抗氧化活性。利用超高效液相色谱-电喷雾串联四级杆质谱仪(UPLC-ESI-MS/MS),对总黄酮含量显著增加的20、40 Gy辐射组与对照组中黄酮类物质进行靶向代谢组学分析。结果表明,10~40 Gy12C^(6+)辐射均提高了菊苣根总黄酮含量,其中20、40 Gy辐射组含量提升最显著;鉴定出5类11种黄酮类代谢物,橙皮素、氯化矢车菊素-3-O-芸香糖苷是响应12C^(6+)辐射致菊苣根总黄酮含量升高的关键差异代谢物,12C^(6+)辐射影响了紫云英苷、异鼠李素-3-O-葡萄糖苷、木犀草素和橙皮素的合成。类黄酮生物合成途径(ko00941)、黄酮和黄酮醇的生物合成途径(ko00944)富集显著。20、40 Gy剂量辐射提高了菊苣根黄酮提取液的1,1-二苯基-2-三硝基苯肼(DPPH)、2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS+)自由基清除能力及铁离子还原能力(FRAP),显著增强了其抗氧化活性。综上,20、40 Gy剂量的12C^(6+)辐射能提高菊苣根中总黄酮含量,改变菊苣根中黄酮类物质种类及含量,并增强其抗氧化活性。本研究结果为进一步研究菊苣辐射诱变育种提供了理论依据。To investigate the effects of 12C^(6+)radiation on flavonoids and antioxidant activity in chicory,chicory roots were used as experimental materials to determine the total flavonoid content and antioxidant activity under different 12C^(6+)radiation doses.Ultra-high performance liquid chromatography-electrospray ionization tandem four-stage rod mass spectrometry(UPLC-ESI-MS/MS)was used to conduct targeted metabonomic analysis of flavonoids in 20 and 40 Gy radiation groups which had significantly increased total flavonoids content and control groups.The results showed that 10-40 Gy 12C^(6+)radiation increased the total flavonoid content of chicory roots,with the most significant increase in the 20 and 40 Gy radiation groups.Five categories of 11 flavonoid metabolites were identified,with hesperetin and cyanidin 3-O-rutinoside chloride being the key differential metabolites in response to the increase in total flavonoid content of chicory roots caused by 12C6+radiation.12C^(6+)radiation affected the synthesis of astragalin,isorhamnetin-3-O-glucoside,luteolin,and hesperetin.Flavonoid biosynthesis pathway(ko00941)and Flavone and flavonol biosynthesis pathway(ko00944)were significantly enriched.20 and 40 Gy radiation increased the DPPH,ABTS+radical scavenging ability,and ferric ion reduction ability(FRAP)of flavonoid extract from chicory root,and significantly enhanced its antioxidant activity.In summary,12C^(6+)radiation at doses of 20 and 40 Gy can increase the total flavonoid content,alter the types and content of flavonoids in chicory roots,and enhance their antioxidant activity.The results of this study provide a theoretical basis for further research on radiation induced breeding of chicory.

关 键 词：菊苣 12C^(6+)辐射 UPLC-ESI-MS/MS 黄酮 代谢组学 

分 类 号：S636.9[农业科学—蔬菜学]