老年急性缺血性脑卒中患者血清miR-340-5p及STING水平与病情及预后的关系  

The Relationship between Seru miR-340-5p, STING Levels andDisease Condition and Prognosis in Elderly Patients with Acute Ischemic Stroke

作　　者：马艳[1] 胡芳 吕学海[1] 刘运平[1] 王晓莹 柴佳 郭一诺 朱晓帅 Ma Yan;Hu Fang;Lv Xuehai;Liu Yunping;Wang Xiaoying;Chai Jia;Guo Yinuo;Zhu Xiaoshuai(Handan Central Hospital,Handan 056000)

机构地区：[1]邯郸市中心医院,邯郸056000

出　　处：《国际老年医学杂志》2024年第4期439-443,共5页International Journal of Geriatrics

基　　金：邯郸市科学技术研究与发展计划项目(22422083027ZC)。

摘　　要：目的 探究老年急性缺血性脑卒中患者血清miR-340-5p及干扰素基因刺激蛋白(STING)水平与病情及预后的关系。方法 选择邯郸市中心医院2020年5月—2022年5月收治的老年急性缺血性脑卒中患者113例作为研究组,另选取同期正常健康者113例作为对照组。根据患者入院当天的美国国立卫生研究院卒中量表(NIHSS)评分评估神经缺损程度(<6分为轻度,6~<14分为中度,≥14分为重度),根据治疗3个月后改良Rankin量表(mRs)评分评估预后[预后良好(0~2分)、预后不良(≥3分)]。采用实时荧光定量PCR法检测血清miR-340-5p、STING表达水平,多因素logistic回归分析预后的影响因素。ROC曲线分析血清miR-340-5p、STING对预后的预测价值。结果 研究组血清miR-340-5p表达水平低于对照组(P<0.05),血清STING表达水平高于对照组(P<0.05)。神经缺损程度轻度37例、中度43例、重度33例,血清miR-340-5p在不同神经缺损程度患者中的表达水平为重度者<中度者<轻度者(P<0.05),而血清STING在不同神经缺损程度患者中的表达水平为重度者>中度者>轻度者(P<0.05)。预后良好74例,预后不良39例,预后不良组血清STING水平、入院时NIHSS评分均高于预后良好组(P<0.05),而血清miR-340-5p水平低于预后良好组(P<0.05)。多因素logistic回归分析结果显示:血清miR-340-5p为预后的保护因素(P<0.05),血清STING及NIHSS评分为其危险因素(P<0.05);ROC曲线显示,血清miR-340-5p、STING联合预测预后的曲线下面积大于各指标单独预测(P<0.05)。结论 血清miR-340-5p及STING表达水平均能反映老年急性缺血性脑卒中患者的病情,在预后预测方面具有一定的临床价值。Objective To investigate the relationship between serum miR-340-5p and stimulator of interferon gene(STING)levels in elderly patients with acute ischemic stroke and their condition and prognosis.Methods A total of 113 elderly patients with acute ischemic stroke admitted to Handan Central Hospital from May 2020 to May 2022 were selected as the study group,and 113 normal healthy patients during the same period were selected as the control group,according to the neurological impairment was assessed according to the National Institutes of Health Stroke Scale(NIHSS)score on the day of admission(<6 scores as mild,6-<14 scores as moderate,≥14 scores as severe),and prognosis was assessed according to the modified Rankin scale(mRs)score after 3 months of treatment[good prognosis(0-2 scores)and poor prognosis(≥3 scores)].The expression levels of serum miR-340-5p and STING were detected by real-time fluorescence quantitative PCR.The influencing factors of prognosis were analyzed by multivariate logistic regression.ROC curve was used to analyze the predictive value of serum miR-340-5p and STING for prognosis.Results The expression level of serum miR-340-5p in study group was lower than that in control group(P<0.05),and the expression level of STING in serum was higher than that in control group(P<0.05).There were 37 cases with mild nerve defects,43 cases with moderate nerve defects,and 33 cases with severe nerve defects.The expression level of serum miR-340-5p in patients with different nerve defects was severe<moderate<mild(P<0.05),while the expression level of serum STING in patients with different nerve defects was severe>moderate>mild(P<0.05).There were 74 patients with good prognosis and 39 patients with poor prognosis.Serum STING level and NIHSS score at admission in the poor prognosis group were higher than those in the good prognosis group(P<0.05),while serum miR-340-5p level was lower than those in the good prognosis group(P<0.05).Multivariate logistic regression analysis showed that serum miR-340-5p was the pro

关 键 词：miR-340-5p 干扰素基因刺激蛋白 急性缺血性脑卒中 预后 

分 类 号：R743.3[医药卫生—神经病学与精神病学]