葫芦巴转录组测序及生物信息学分析  

Transcriptome Sequencing and Bioinformatics Analysis of Trigonella foenum-graceum

作　　者：王星哲 陈阳 武悦[1] 单飞彪[1] 杜瑞霞[1] 闫文芝[1] 刘春晖[1] Wang Xingzhe;Chen Yang;Wu Yue;Shan Feibiao;Du Ruixia;Yan Wenzhi;Liu Chunhui(Agricultural Biotechnology Research Center,Bayannur Academy of Agricultural and Animal Sciences,Bayannur,015000;College of Agronomy,Hunan Agricultural University,Changsha,410000)

机构地区：[1]巴彦淖尔市农牧业科学研究院,农业生物技术研究中心,巴彦淖尔015000 [2]湖南农业大学农学院,长沙410000

出　　处：《分子植物育种》2024年第13期4254-4261,共8页Molecular Plant Breeding

基　　金：内蒙古自治区科技成果转化专项(BZC191033);内蒙古农牧业科学院青年创新基金项目(2020QNJJNO15)共同资助。

摘　　要：本研究基于RNA-Seq技术对葫芦巴幼苗叶片进行转录组测序及生物信息学分析。获得葫芦巴转录组数据,为后续研究葫芦巴基因功能、代谢途径、分子标记及调控机理等方面提供参考。对测序数据拼接组装后获得16 456条unigene,总长度为19 770 394 bp。将获得的unigene分别与KEGG、Nr、GO和KOG等7大数据库进行比对,共有11 768条unigene得到注释,占总unigene的71.62%。其中GO数据库注释unigene9 029条,按照功能分为3个大类和52个亚类;有8 158条unigene在KEGG数据库中得到注释,涉及代谢通路300条;11 658条unigene在Nr数据库中获得注释,注释的同源序列主要来自豆科植物蒺藜苜蓿;有6 717条unigene在KOG数据库得到注释,涉及25个不同的功能分类。葫芦巴所有unigene中共检索到11 286个SNP位点,以C:G>T:A和T:A>C:G的突变类型发生频率最高;SSR特征分析中共检测到4 928个位点,其中单核苷酸重复2 262个,占SSR位点总数的45.90%。本研究构建葫芦巴转录组数据库并进行生物信息学分析,为后续开展种质资源鉴定、遗传多样性分析、指纹图谱构建等研究提供科学依据。Transcriptome sequencing and bioinformatics analysis of Trigonella foenum-graceum were performed based on RNA-Seq technology.The transcriptome data of Trigonella foenum-graceum were obtained to provide reference for future studies on the gene function,metabolic pathway,molecular marker and regulatory mechanism of Trigonella foenum-graceum.A total of 16456 unigenes with a total length of 19770394 bp were obtained from the transcriptome data of Trigonella foenum-graceum.By comparing the obtained unigenes with seven databases,such as KEGG,Nr,GO and KOG,11768 unigenes were annotated,accounting for 71.62%of the total unigenes.9029 unigenes were annotated in GO database and divided into 3 categories and 52 subcategories according to their functions.There were 8158 annotated unigenes in KEGG database,which were involved in 300 metabolic pathways.In addition,11658 unigene were annotated in the Nr database,and the annotated homologous sequences were mainly from Medicago truncatula.In KOG database,6717 unigenes were annotated into 25 different functional classifications.A total of 11286 SNP loci were identificated from the transcriptome data,among which C:G>T:A and T:A>C:G were the highest frequent mutation types.A total of 4928 SSR loci were detected,and the single nucleotide repeats(2262)accounted for 45.90%of the total.In this study,Trigonella foenum-graceum transcriptome database was constructed and bioinformatics analysis was performed,which provided scientific basis for identification of germplasm resources,analysis of genetic diversity and construction of fingerprint map of Trigonella foenum-graceum.

关 键 词：葫芦巴 转录组 UNIGENE 分子标记 

分 类 号：S567.219[农业科学—中草药栽培]